Proteintech

RFC2 Polyclonal Antibody

RFC2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

RFC2 Antibody (10410-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10410-1-AP (RFC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RFC2 Antibody in Immunoprecipitation (IP)

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Product Details

10410-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RFC2 Fusion Protein Ag0663 (1-320 aa encoded by BC002813)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEVEAVCGG AGEVEAQDSD PAPAFSKAPG SAGHYELPWV EKYRPVKLNE IVGNEDTVSR LEVFAREGNV PNIIIAGPPG TGKTTSILCL ARALLGPALK DAMLELNASN DSMTDGAQQA LRRTMEIYSK TTRFALACNA SDKIIEPIQS RCAVLRYTKL TDAQILTRLM NVIEKERVPY TDDGLEAIIF TAQGDMRQAL NNLQSTFSGF GFINSENVFK VCDEPHPLLV KEMIQHCVNA NIDEAYKILA HLWHLGYSPE DIIGNIFRVC KTFQMAEYLK LEFIKEIGYT HMKIAEGVNS LLQMAGLLAR LCQKTMAPVA S (1-320 aa encoded by BC002813)

Target Information

This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination.

bioRxiv : the preprint server for biology

Wang YC,Kelso AA,Karamafrooz A,Chen YH,Chen WK,Cheng CT,Qi Y,Gu L,Malkas L,Kung HJ,Moldovan GL,Ciccia A,Stark JM,Ann DK

Fri Feb 03 00:00:00 UTC 2023

Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination.

Cell reports

Wang YC,Kelso AA,Karamafrooz A,Chen YH,Chen WK,Cheng CT,Qi Y,Gu L,Malkas L,Taglialatela A,Kung HJ,Moldovan GL,Ciccia A,Stark JM,Ann DK

Tue Apr 25 00:00:00 UTC 2023

RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.

Scientific reports

Zhao X,Wang Y,Li J,Qu F,Fu X,Liu S,Wang X,Xie Y,Zhang X

Thu Feb 24 00:00:00 UTC 2022

The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.

Molecular systems biology

Caron E,Vincent K,Fortier MH,Laverdure JP,Bramoullé A,Hardy MP,Voisin G,Roux PP,Lemieux S,Thibault P,Perreault C

Tue Sep 27 00:00:00 UTC 2011

The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.

Molecular systems biology

Caron E,Vincent K,Fortier MH,Laverdure JP,Bramoullé A,Hardy MP,Voisin G,Roux PP,Lemieux S,Thibault P,Perreault C

Tue Sep 27 00:00:00 UTC 2011

Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.

Proceedings of the National Academy of Sciences of the United States of America

Chiang PM,Ling J,Jeong YH,Price DL,Aja SM,Wong PC

Tue Sep 14 00:00:00 UTC 2010

Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.

Proceedings of the National Academy of Sciences of the United States of America

Chiang PM,Ling J,Jeong YH,Price DL,Aja SM,Wong PC

Tue Sep 14 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: A1 40 kDa subunit; Activator 1 40 kDa subunit; Activator 1 subunit 2; Activator 1 subunit C2; MGC3665; replication factor C (activator 1) 2, 40kDa; Replication factor C 40 kDa subunit; Replication factor C subunit 2; replication factor C, 40 kDa; RF-C 40 kDa subunit; RFC40

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Gene Aliases: 2610008M13Rik; 40kDa; AI326953; Recc2; RFC2; RFC40

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UniProt ID: (Human) P35250, (Mouse) Q9WUK4, (Rat) Q641W4

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Entrez Gene ID: (Human) 5982, (Mouse) 19718, (Rat) 116468

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Function(s)

DNA clamp loader activity protein binding ATP binding enzyme binding single-stranded DNA-dependent ATPase activity nucleotide binding DNA binding molecular_function

Process(es)

telomere maintenance via recombination DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling translesion synthesis nucleotide-excision repair, DNA incision error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis positive regulation of DNA-directed DNA polymerase activity regulation of signal transduction by p53 class mediator DNA-dependent DNA replication biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RFC2 Polyclonal Antibody

RFC2 Antibody (10410-1-AP) in ICC/IF

Product Details

10410-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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