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Invitrogen
RFK Polyclonal Antibody
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FIGURE: 1 / 1
RFK Antibody (PA5-97187) in WB
Western blot analysis of RFK in extracts of various cell lines. Samples were incubated with RFK Polyclonal antibody (Product # PA5-97187) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-97187
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_0608093)
Conjugate
Form
Liquid
Concentration
1.81 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808989
Product Specific Information
Immunogen sequence: MRHLPYFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DISTGIYYGW ASVGSGDVHK MVVSIGWNPY YKNTKKSMET HIMHTFKEDF YGEILNVAIV GYLRPEKNFD SLESLISAIQ GDIEEAKKRL ELPEHLKIKE DNFFQVSKSK IMNGH; Positive Samples: U-87MG, HeLa, 293T, Mouse small intestine, Mouse brain; Cellular Location: Cytoplasm
Target Information
Riboflavin kinase is an essential enzyme that catalyzes the phosphorylation of riboflavin to form flavin mononucleotide, an obligatory step in vitamin B2 utilization and flavin cofactor synthesis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 0610038L10Rik; ATP:riboflavin 5'-phosphotransferase; Flavokinase; KOI-4; Riboflavin kinase
Gene Aliases: 0610038L10Rik; AF031381; KOI-4; RFK; RIFK
UniProt ID: (Human) Q969G6, (Mouse) Q8CFV9
Entrez Gene ID: (Human) 55312, (Mouse) 54391
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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