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Proteintech
RFWD3 Polyclonal Antibody
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Product Details
19893-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RFWD3 Fusion Protein Ag13728 (427-774 aa encoded by BC002574)
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
From BLAST result, this antibody is specific to RFWD3.45 kDa and 55 kDa maybe spliceosome of RFWD3.
Immunogen sequence: SQGQHKHKY HFQKTFTVSQ AGNCRIMAYC DALSCLVISQ PSPQASFLPG FGVKMLSTAN MKSSQYIPMH GKQIRGLAFS SYLRGLLLSA SLDNTIKLTS LETNTVVQTY NAGRPVWSCC WCLDEANYIY AGLANGSILV YDVRNTSSHV QELVAQKARC PLVSLSYMPR AASAAFPYGG VLAGTLEDAS FWEQKMDFSH WPHVLPLEPG GCIDFQTENS SRHCLVTYRP DKNHTTIRSV LMEMSYRLDD TGNPICSCQP VHTFFGGPTC KLLTKNAIFQ SPENDGNILV CTGDEAANSA LLWDAASGSL LQDLQTDQPV LDICPFEVNR NSYLATLTEK MVHIYKWE (427-774 aa encoded by BC002574)
Target Information
E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RFWD3; ring finger and WD repeat domain 3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201
Gene Aliases: BC027246; RFWD3; RGD1305243; RNF201
UniProt ID: (Human) Q6PCD5, (Mouse) Q8CIK8
Entrez Gene ID: (Human) 55159, (Mouse) 234736, (Rat) 361409
p53 binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
MDM2/MDM4 family protein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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