Proteintech

RGS14 Polyclonal Antibody

RGS14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RGS14 Antibody (16258-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 16258-1-AP) (RGS14 antibody) at dilution of 1:5,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RGS14 Antibody in Immunoprecipitation (IP)

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Product Details

16258-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RGS14 Fusion Protein Ag9292 (217-566 aa encoded by BC014094)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KSLPLGVEE LGQLPPVEGP GGRPLRKSFR RELGGTANAA LRRESQGSLN SSASLDLGFL AFVSSKSESH RKSLGSTEGE SESRPGKYCC VYLPDGTASL ALARPGLTIR DMLAGICEKR GLSLPDIKVY LVGNEQALVL DQDCTVLADQ EVRLENRITF ELELTALERV VRISAKPTKR LQEALQPILE KHGLSPLEVV LHRPGEKQPL DLGKLVSSVA AQRLVLDTLP GVKISKARDK SPCRSQGCPP RTQDKATHPP PASPSSLVKV PSSATGKRQT CDIEGLVELL NRVQSSGAHD QRGLLRKEDL VLPEFLQLPA QGPSSEETPP QTKSAAQPIG GSLNSTTDSA L (217-566 aa encoded by BC014094)

Target Information

RGS14 is a member of the regulator of G-protein signaling family which contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. RGS14 attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. The resulting hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of RGS14 have been observed but have not been thoroughly characterized. Further, RGS14 acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism; plays a role in cell division; is required for the nerve growth factor (NGF)-mediated neurite outgrowth, and is involved in stress resistance. RGS14 protein may also be involved in visual memory processing capacity and hippocampal-based learning and memory.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Defining hippocampal area CA2 in the fox (Vulpes vulpes) brain.

Hippocampus

Dudek SM,Phoenix AN,Scappini E,Shepeleva DV,Herbeck YE,Trut LN,Farris S,Kukekova AV

Thu Jun 01 00:00:00 UTC 2023

RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.

The Journal of biological chemistry

Friedman PA,Sneddon WB,Mamonova T,Montanez-Miranda C,Ramineni S,Harbin NH,Squires KE,Gefter JV,Magyar CE,Emlet DR,Hepler JR

Sun May 01 00:00:00 UTC 2022

RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.

The Journal of biological chemistry

Friedman PA,Sneddon WB,Mamonova T,Montanez-Miranda C,Ramineni S,Harbin NH,Squires KE,Gefter JV,Magyar CE,Emlet DR,Hepler JR

Sun May 01 00:00:00 UTC 2022

Human genetic variants disrupt RGS14 nuclear shuttling and regulation of LTP in hippocampal neurons.

The Journal of biological chemistry

Squires KE,Gerber KJ,Tillman MC,Lustberg DJ,Montañez-Miranda C,Zhao M,Ramineni S,Scharer CD,Saha RN,Shu FJ,Schroeder JP,Ortlund EA,Weinshenker D,Dudek SM,Hepler JR

Fri Aug 27 00:00:00 UTC 2021

Human genetic variants disrupt RGS14 nuclear shuttling and regulation of LTP in hippocampal neurons.

The Journal of biological chemistry

Squires KE,Gerber KJ,Tillman MC,Lustberg DJ,Montañez-Miranda C,Zhao M,Ramineni S,Scharer CD,Saha RN,Shu FJ,Schroeder JP,Ortlund EA,Weinshenker D,Dudek SM,Hepler JR

Fri Aug 27 00:00:00 UTC 2021

Modelling cognitive deficits in Parkinson's disease: Is CA2 a gateway for hippocampal synucleinopathy?

Experimental neurology

Cinar E,Yalcin-Cakmakli G,Saka E,Ulusoy A,Yuruker S,Elibol B,Tel BC

Sat Aug 01 00:00:00 UTC 2020

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.

Brain structure & function

Squires KE,Gerber KJ,Pare JF,Branch MR,Smith Y,Hepler JR

Mon Jan 01 00:00:00 UTC 2018

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.

Brain structure & function

Squires KE,Gerber KJ,Pare JF,Branch MR,Smith Y,Hepler JR

Mon Jan 01 00:00:00 UTC 2018

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.

Brain structure & function

Squires KE,Gerber KJ,Pare JF,Branch MR,Smith Y,Hepler JR

Mon Jan 01 00:00:00 UTC 2018

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.

Brain structure & function

Squires KE,Gerber KJ,Pare JF,Branch MR,Smith Y,Hepler JR

Mon Jan 01 00:00:00 UTC 2018

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.

Brain structure & function

Squires KE,Gerber KJ,Pare JF,Branch MR,Smith Y,Hepler JR

Mon Jan 01 00:00:00 UTC 2018

14-3-3γ binds regulator of G protein signaling 14 (RGS14) at distinct sites to inhibit the RGS14:Gα_i-AlF₄^- signaling complex and RGS14 nuclear localization.

The Journal of biological chemistry

Gerber KJ,Squires KE,Hepler JR

Fri Sep 21 00:00:00 UTC 2018

14-3-3γ binds regulator of G protein signaling 14 (RGS14) at distinct sites to inhibit the RGS14:Gα_i-AlF₄^- signaling complex and RGS14 nuclear localization.

The Journal of biological chemistry

Gerber KJ,Squires KE,Hepler JR

Fri Sep 21 00:00:00 UTC 2018

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

PloS one

Branch MR,Hepler JR

Wed Oct 18 00:00:00 UTC 2017

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

PloS one

Branch MR,Hepler JR

Wed Oct 18 00:00:00 UTC 2017

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

PloS one

Branch MR,Hepler JR

Wed Oct 18 00:00:00 UTC 2017

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

PloS one

Branch MR,Hepler JR

Wed Oct 18 00:00:00 UTC 2017

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

PloS one

Branch MR,Hepler JR

Wed Oct 18 00:00:00 UTC 2017

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

PloS one

Branch MR,Hepler JR

Wed Oct 18 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: rap1/rap2 interacting protein; RAP1/RAP2-interacting protein; Regulator of G-protein signaling 14; regulator of G-protein signalling 14; RGS14; RGSE; RPIP1

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Gene Aliases: RGS14; RPIP1

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UniProt ID: (Human) O43566, (Mouse) P97492

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Entrez Gene ID: (Human) 10636, (Mouse) 51791

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Function(s)

G-protein alpha-subunit binding receptor signaling protein activity GDP-dissociation inhibitor activity GTPase activator activity protein binding microtubule binding protein kinase binding receptor signaling complex scaffold activity GTPase activating protein binding GTPase regulator activity

Process(es)

nucleocytoplasmic transport response to oxidative stress cell cycle spindle organization chromosome segregation mitotic nuclear division signal transduction G-protein coupled receptor signaling pathway multicellular organism development learning long-term memory regulation of G-protein coupled receptor protein signaling pathway visual learning negative regulation of signal transduction zygote asymmetric cell division negative regulation of synaptic plasticity intracellular signal transduction termination of G-protein coupled receptor signaling pathway negative regulation of MAP kinase activity positive regulation of GTPase activity regulation of DNA-templated transcription in response to stress platelet-derived growth factor receptor signaling pathway positive regulation of neurogenesis cell division long-term synaptic potentiation negative regulation of ERK1 and ERK2 cascade

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RGS14 Polyclonal Antibody

RGS14 Antibody (16258-1-AP) in IHC (P)

Product Details

16258-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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