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RGS6 Antibody (PA5-112692) in ICC/IF
Immunocytochemichal analysis of RGS6 in Hela cells using a Polyclonal antibody (Product # PA5-112692) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-112692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Regulator of G-protein signaling 6 protein (177-262AA)
Conjugate
Form
Liquid
Concentration
4.02 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2867427
Target Information
Members of the RGS (regulator of G protein signaling) family, such as RGS6, modulate G protein function by activating the intrinsic GTPase activity of the alpha (guanine nucleotide-binding) subunits (Seki et al., 1999 [PubMed 10083744]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp313G1241; FLJ43552; H_DJ1108A12.1; MGC142132; Regulator of G-protein signaling 6; regulator of G-protein signalling 6; RGS6; S914; WUGSC:H_DJ0283M22.1; WUGSC:H_DJ1108A12.1
Gene Aliases: GAP; RGS6
UniProt ID: (Human) P49758
Entrez Gene ID: (Human) 9628, (Rat) 54295
G-protein coupled receptor signaling pathway
regulation of G-protein coupled receptor protein signaling pathway
negative regulation of signal transduction
intracellular signal transduction
positive regulation of GTPase activity
termination of G-protein coupled receptor signaling pathway
positive regulation of neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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