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Invitrogen
RHBDD1 Polyclonal Antibody
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RHBDD1 Antibody (PA5-20860) in WB
Western Blot analysis in A549 Cells. Loading: 15 µg of cell lysate. Antibodies: RHBDD1 Polyclonal Antibody (Product # PA5-20860) 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Exposure: 1 min.
Product Details
PA5-20860
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 14 amino acid peptide from near the carboxy terminus of human RHBDD1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11157222
Product Specific Information
A suggested positive control is K562 cell lysate.
PA5-20860 can be used with blocking peptide PEP-0974.
Target Information
The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mRHBDD1; Rhomboid domain-containing protein 1; Rhomboid-like protein 4; Rhomboid-related protein 4; RRP4
Gene Aliases: 4930418P06Rik; HSD-50; HSD50; mRHBDD1; MSD-50; MSD50; RHBDD1; RHBDL4; RRP4
UniProt ID: (Human) Q8TEB9, (Mouse) Q8BHC7
Entrez Gene ID: (Human) 84236, (Mouse) 76867
proteolysis
apoptotic process
spermatogenesis
positive regulation of protein processing
cell differentiation
ER-associated ubiquitin-dependent protein catabolic process
membrane protein intracellular domain proteolysis
membrane protein proteolysis
cellular response to unfolded protein
cellular response to UV
negative regulation of apoptotic process
post-translational protein modification
positive regulation of protein catabolic process
spermatid differentiation
positive regulation of secretion
regulation of male germ cell proliferation
ERAD pathway
membrane protein proteolysis involved in retrograde protein transport, ER to cytosol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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