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Invitrogen
RHD Chimeric Recombinant Rabbit Monoclonal Antibody (D7C2)
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FIGURE: 1 / 2
RHD Chimeric Antibody (MA5-48188) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of RHD in K562 cells using RHD Chimeric Monoclonal Antibody (Product # MA5-48188). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with RHD Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, t... View More
Product Details
MA5-48188
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
D7C2
Immunogen
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074631
Target Information
The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans. Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1-4). These tightly linked genes map to human chromosomal position 1p34.1-1p36. The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2,5). Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1,2,6). The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2,4,5). The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity. The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: blood group antigen D; blood group protein RHD; Blood group Rh(D) polypeptide; CD240D; D antigen (DCS); Rh blood group antigen Evans; Rh blood group, D anitgen; Rh blood group, D antigen; RH polypeptide 2; RhD blood group antigen; RhD polypeptide; Rhesus blood group D antigen allele DIII type 7; Rhesus D antigen; Rhesus system D polypeptide; RhPII; RHXIII; truncated rhesus blood group D antigen; truncated rhesus D
Gene Aliases: CD240D; DIIIc; RH; RH30; Rh4; RHCED; RHD; RhDCw; RHDel; RHDVA(TT); RhII; RhK562-II; RhPI; RHPII; RHXIII
UniProt ID: (Human) Q02161
Entrez Gene ID: (Human) 6007
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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