Proteintech

RHEB Polyclonal Antibody

RHEB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RHEB Antibody (15924-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human brain tissue slide using 15924-1-AP ( RHEB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

RHEB Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

15924-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RHEB Fusion Protein Ag8747 (1-184 aa encoded by BC016155)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPQSKSRKI AILGYRSVGK SSLTIQFVEG QFVDSYDPTI ENTFTKLITV NGQEYHLQLV DTAGQDEYSI FPQTYSIDIN GYILVYSVTS IKSFEVIKVI HGKLLDMVGK VQIPIMLVGN KKDLHMERVI SYEEGKALAE SWNAAFLESS AKENQTAVDV FRRIILEAEK MDGAASQGKS SCSVM (1-184 aa encoded by BC016155)

Target Information

Rheb encodes a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Stem cell research & therapy

Jin C,Ou Q,Chen J,Wang T,Zhang J,Wang Z,Wang Y,Tian H,Xu JY,Gao F,Wang J,Li J,Lu L,Xu GT

Fri Apr 01 00:00:00 UTC 2022

PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.

Frontiers in cell and developmental biology

Luo Q,Li X,Gan G,Yang M,Chen X,Chen F

Sat Jul 16 00:00:00 UTC 2022

Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.

Metabolism: clinical and experimental

Yang Y,Cai Z,Pan Z,Liu F,Li D,Ji Y,Zhong J,Luo H,Hu S,Song L,Yu S,Li T,Li J,Ma X,Zhang W,Zhou Z,Liu F,Zhang J

Fri Oct 01 00:00:00 UTC 2021

MYH9 is crucial for stem cell-like properties in non-small cell lung cancer by activating mTOR signaling.

Cell death discovery

Chen M,Sun LX,Yu L,Liu J,Sun LC,Yang ZH,Shu X,Ran YL

Mon Oct 11 00:00:00 UTC 2021

RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.

PLoS biology

Proietti Onori M,Koene LMC,Schäfer CB,Nellist M,de Brito van Velze M,Gao Z,Elgersma Y,van Woerden GM

Sat May 01 00:00:00 UTC 2021

Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells.

Cell death & disease

Chen X,Zhang D,Wang Y,Chen K,Zhao L,Xu Y,Jiang H,Wang S

Thu May 28 00:00:00 UTC 2020

A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II.

Experimental & molecular medicine

Zhao S,Li Z,Zhang M,Zhang L,Zheng H,Ning J,Wang Y,Wang F,Zhang X,Gan H,Wang Y,Zhang X,Luo H,Bu G,Xu H,Yao Y,Zhang YW

Tue Jul 23 00:00:00 UTC 2019

CircRNA-100338 Is Associated With mTOR Signaling Pathway and Poor Prognosis in Hepatocellular Carcinoma.

Frontiers in oncology

Huang XY,Huang ZL,Zhang PB,Huang XY,Huang J,Wang HC,Xu B,Zhou J,Tang ZY

Thu Oct 01 00:00:00 UTC 2020

Ox-LDL-Induced MicroRNA-155 Promotes Autophagy in Human Endothelial Cells via Repressing the Rheb/ mTOR Pathway.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Lv J,Yang L,Guo R,Shi Y,Zhang Z,Ye J

Mon Dec 18 00:00:00 UTC 2017

Ox-LDL-Induced MicroRNA-155 Promotes Autophagy in Human Endothelial Cells via Repressing the Rheb/ mTOR Pathway.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Lv J,Yang L,Guo R,Shi Y,Zhang Z,Ye J

Mon Dec 18 00:00:00 UTC 2017

Rheb phosphorylation is involved in p38-regulated/activated protein kinase-mediated tumor suppression in liver cancer.

Oncology letters

Zheng M,Zang S,Xie L,Fang X,Zhang YU,Ma X,Liu J,Lin D,Huang A

Tue Sep 01 00:00:00 UTC 2015

Rheb phosphorylation is involved in p38-regulated/activated protein kinase-mediated tumor suppression in liver cancer.

Oncology letters

Zheng M,Zang S,Xie L,Fang X,Zhang YU,Ma X,Liu J,Lin D,Huang A

Tue Sep 01 00:00:00 UTC 2015

Rheb phosphorylation is involved in p38-regulated/activated protein kinase-mediated tumor suppression in liver cancer.

Oncology letters

Zheng M,Zang S,Xie L,Fang X,Zhang YU,Ma X,Liu J,Lin D,Huang A

Tue Sep 01 00:00:00 UTC 2015

Rheb phosphorylation is involved in p38-regulated/activated protein kinase-mediated tumor suppression in liver cancer.

Oncology letters

Zheng M,Zang S,Xie L,Fang X,Zhang YU,Ma X,Liu J,Lin D,Huang A

Tue Sep 01 00:00:00 UTC 2015

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

Cell metabolism

Shimizu N,Yoshikawa N,Ito N,Maruyama T,Suzuki Y,Takeda S,Nakae J,Tagata Y,Nishitani S,Takehana K,Sano M,Fukuda K,Suematsu M,Morimoto C,Tanaka H

Wed Feb 02 00:00:00 UTC 2011

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

Cell metabolism

Shimizu N,Yoshikawa N,Ito N,Maruyama T,Suzuki Y,Takeda S,Nakae J,Tagata Y,Nishitani S,Takehana K,Sano M,Fukuda K,Suematsu M,Morimoto C,Tanaka H

Wed Feb 02 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: GTP-binding protein Rheb; Ras homolog enriched in brain; Ras homolog enriched in brain 2; RAS-homolog enriched in brain

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Gene Aliases: RHEB; Rheb1; RHEB2

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UniProt ID: (Human) Q15382, (Mouse) Q921J2

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Entrez Gene ID: (Human) 6009, (Mouse) 19744

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Function(s)

GTPase activity GTP binding protein kinase binding metal ion binding nucleotide binding GDP binding

Process(es)

signal transduction small GTPase mediated signal transduction metabolic process positive regulation of TOR signaling regulation of type B pancreatic cell development cell cycle arrest macroautophagy positive regulation of oligodendrocyte differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RHEB Polyclonal Antibody

RHEB Antibody (15924-1-AP) in IHC (P)

Product Details

15924-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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