Invitrogen

RHOT1 Polyclonal Antibody

RHOT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RHOT1 Antibody (PA5-42646) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human muscle tissue using an anti-RHOT1 polyclonal antibody (Product # PA5-42646).

RHOT1 Antibody in Surface plasmon resonance (SPR)

RHOT1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

RHOT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK 293 cells were transfected with RHOT1 siRNA and reduction of signal was observed in Western Blot using RHOT1 Polyclonal Antibody (Product # PA5-42646). [Note: Non-specific band observed at ~35Kda]. {KD}

Product Details

PA5-42646

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Surface plasmon resonance (SPR)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human RHOT1 (aa 1-50).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2606816

Product Specific Information

Peptide sequence: MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Target Information

Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1.

Frontiers in molecular biosciences

Fagbadebo FO,Kaiser PD,Zittlau K,Bartlick N,Wagner TR,Froehlich T,Jarjour G,Nueske S,Scholz A,Traenkle B,Macek B,Rothbauer U

PA5-42646 was used in Western Blot to present a collection of novel Nbs that serve as a toolkit for advanced biochemical and intracellular studies and modulations of Miro1, thereby contributing to the understanding of the functional role of Miro1 in disease-derived model systems.

Sat Apr 02 00:00:00 UTC 2022

A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1.

Frontiers in molecular biosciences

Fagbadebo FO,Kaiser PD,Zittlau K,Bartlick N,Wagner TR,Froehlich T,Jarjour G,Nueske S,Scholz A,Traenkle B,Macek B,Rothbauer U

Sat Apr 02 00:00:00 UTC 2022

A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1.

Frontiers in molecular biosciences

Fagbadebo FO,Kaiser PD,Zittlau K,Bartlick N,Wagner TR,Froehlich T,Jarjour G,Nueske S,Scholz A,Traenkle B,Macek B,Rothbauer U

Sat Apr 02 00:00:00 UTC 2022

Mitochondrial transfer from mesenchymal stem cells improves neuronal metabolism after oxidant injury in vitro: The role of Miro1.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Tseng N,Lambie SC,Huynh CQ,Sanford B,Patel M,Herson PS,Ormond DR

PA5-42646 was used in Western Blot to examine mitochondrial transfer from mesenchymal stem cells to neurons and the role it plays in neuronal preservation after oxidant injury.

Thu Apr 01 00:00:00 UTC 2021

Mitochondrial transfer from mesenchymal stem cells improves neuronal metabolism after oxidant injury in vitro: The role of Miro1.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Tseng N,Lambie SC,Huynh CQ,Sanford B,Patel M,Herson PS,Ormond DR

PA5-42646 was used in Western Blot to examine mitochondrial transfer from mesenchymal stem cells to neurons and the role it plays in neuronal preservation after oxidant injury.

Thu Apr 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: MIRO-1; mitochondrial Rho (MIRO) GTPase 1; Mitochondrial Rho GTPase 1; rac-GTP binding protein-like protein; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1; ras homolog gene family, member T1

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Gene Aliases: ARHT1; MIRO-1; MIRO1; RHOT1

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UniProt ID: (Human) Q8IXI2

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Entrez Gene ID: (Human) 55288

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Function(s)

molecular_function GTPase activity calcium ion binding protein binding GTP binding

Process(es)

small GTPase mediated signal transduction cellular homeostasis mitochondrion transport along microtubule regulation of small GTPase mediated signal transduction mitochondrial outer membrane permeabilization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RHOT1 Polyclonal Antibody

RHOT1 Antibody (PA5-42646) in IHC (P)

Product Details

PA5-42646

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Surface plasmon resonance (SPR)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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