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RIAM Antibody (PA5-78787) in ICC/IF
Immunocytochemistry analysis of APBB1IP using anti-APBB1IP antibody (Product # PA5-78787). APBB1IP was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-APBB1IP antibody (Product # PA5-78787) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with ... View More
Product Details
PA5-78787
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP (647-666aa EQDFMSDLMKALQKKRGNVS).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745903
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human THP-1 whole cell, Jurkat whole cell, human Raji whole cell, U937 whole cell, rat thymus tissue, mouse thymus tissue. IHC: Human Tonsil Tissue, rat lymphaden tissue IHC-F: rat spleen tissue. ICC/IF: A431 cell, JURKAT Cell. Flow: SiHa cell.
Target Information
RIAM appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein; Amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; PREL-1; proline rich EVH1 ligand 1; Proline-rich EVH1 ligand 1; Proline-rich protein 48; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; Rap1-GTP-interacting adaptor molecule; Rap1-interacting adaptor molecule; RARP-1; Retinoic acid-responsive proline-rich protein 1; RIAM; RIAMRARP-1
Gene Aliases: 9930118P07Rik; APBB1IP; INAG1; PREL1; Prp48; RARP1; RIAM
UniProt ID: (Human) Q7Z5R6, (Mouse) Q8R5A3
Entrez Gene ID: (Human) 54518, (Rat) 307171, (Mouse) 54519
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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