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Rockland
RING1B Polyclonal Antibody
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FIGURE: 1 / 2
RING1B Antibody (600-101-292) in ICC/IF
Immunofluorescence Microscopy of Goat anti-RING1B antibody. Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear cou... View More
Product Details
600-101-292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Chimpanzee,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Affinity purified Anti-RING1B antibody is directed against human RING1B protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.
Target Information
RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAP 1; DinG protein; E3 ubiquitin-protein ligase RING2; GS1-120K12.1; HIP2-interacting protein 3; HIPI 3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; Protein DinG; RING 1B; RING 2; RING finger protein 1B; RING finger protein 2; RING finger protein BAP-1; RING-type E3 ubiquitin transferase RING2; RING1b; RNF 2; RNF2
Gene Aliases: AI326319; AI450156; AU019207; BAP-1; BAP1; BOS_16138; DING; HIPI3; RING1B; RING2; RNF2
UniProt ID: (Human) Q99496, (Mouse) Q9CQJ4
Entrez Gene ID: (Human) 6045, (Bovine) 540090, (Dog) 480042, (Rat) 304850, (Mouse) 19821
chromatin binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
RING-like zinc finger domain binding
molecular_function
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
gastrulation with mouth forming second
transcription, DNA-templated
germ cell development
anterior/posterior axis specification
protein sumoylation
spinal cord development
response to retinoic acid
histone H2A monoubiquitination
histone H2A-K119 monoubiquitination
negative regulation of sequence-specific DNA binding transcription factor activity
histone ubiquitination
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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