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Invitrogen
RIP140 Polyclonal Antibody
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FIGURE: 1 / 4
RIP140 Antibody (PA1-841) in ICC/IF
Immunofluorescence analysis of RIP140 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RIP140 Polyclonal Antibody (Product # PA1-841) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731... View More
Product Details
PA1-841
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2154941
Product Specific Information
PA1-841 detects recombinant human Receptor-Interacting Protein 140 (RIP 140).
PA1-841 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects two proteins at ~140 kDa representing human RIP 140 expressed in rabbit reticulocyte lysate.
The PA1-841 immunogen is a synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140. This immunizing peptide (Cat. # PEP-030) is available for use in neutralization and control experiments.
Target Information
Nuclear receptor interacting protein 1 is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ERAP 140; FLJ77253; Nuclear factor RIP140; Nuclear receptor-interacting protein 1; OTTHUMP00000083036; OTTHUMP00000083037; Receptor-interacting protein 140
Gene Aliases: NRIP1; RIP140
UniProt ID: (Human) P48552
Entrez Gene ID: (Human) 8204
core promoter sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
protein binding
estrogen receptor binding
nuclear hormone receptor binding
glucocorticoid receptor binding
histone deacetylase binding
retinoid X receptor binding
androgen receptor binding
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle rupture
transcription, DNA-templated
circadian rhythm
lipid storage
androgen receptor signaling pathway
circadian regulation of gene expression
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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