OriGene

RIPK1 Monoclonal Antibody (OTI2D6), TrueMAB™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF800312

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2D6

Immunogen

Human recombinant protein fragment corresponding to amino acids 133-422 of human RIPK1 produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell death protein RIP; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; Receptor-interacting protein 1; receptor-interacting protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; RIP-1; RIP1 (CT); serine/threonine-protein kinase RIP

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Gene Aliases: RIP; RIP-1; RIP1; RIPK1

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UniProt ID: (Human) Q13546

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Entrez Gene ID: (Human) 8737

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Function(s)
protein kinase activity protein serine/threonine kinase activity death receptor binding protein binding ATP binding ubiquitin protein ligase binding protein complex binding identical protein binding death domain binding
Process(es)
positive regulation of protein phosphorylation apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process I-kappaB kinase/NF-kappaB signaling activation of JUN kinase activity regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death positive regulation of necrotic cell death positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production tumor necrosis factor-mediated signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway peptidyl-serine autophosphorylation positive regulation of apoptotic process positive regulation of programmed cell death positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling cellular protein catabolic process positive regulation of macrophage differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade protein autophosphorylation positive regulation of NF-kappaB transcription factor activity protein homooligomerization protein heterooligomerization positive regulation of necroptotic process T cell apoptotic process necroptotic process regulation of ATP:ADP antiporter activity cellular response to tumor necrosis factor cellular response to growth factor stimulus death-inducing signaling complex assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway ripoptosome assembly necroptotic signaling pathway ripoptosome assembly involved in necroptotic process regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of hydrogen peroxide-induced cell death amyloid fibril formation positive regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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