Invitrogen

RNA pol II CTD Monoclonal Antibody

1 Published Figure

2 References

View all (46) RNA pol II CTD antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (1)

RNA pol II CTD Antibody in ChIP-Sequencing (ChIP-seq)

FIGURE: 1 / 7

RNA pol II CTD Antibody (49-1033) in ChIP-seq

ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 2 µL of the anti-Pol II antibody (Product # 49-1033). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the enrichment ... View More

RNA pol II CTD Antibody in Immunocytochemistry (ICC/IF)

RNA pol II CTD Antibody in Western Blot (WB)

RNA pol II CTD Antibody in ChIP Assay (ChIP)

Product Image Gallery

Product Details

49-1033

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunocytochemistry (ICC/IF)

1:100

ChIP assay (ChIP)

1-10 µL/1x10^6 cells

View 2 publications 2 publications

ChIP-sequencing (ChIP-Seq)

2 µL/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Xenopus

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Immunogen

Raised against the largest subunit of RNA polymerase II of wheat germ and interacts with the highly conserved C-terminal domain of this protein containing the repeat YSPTSPS.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

<0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533882

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells.

Cell death & disease

Chao HH,Luo JL,Hsu MH,Chen LH,Lu TP,Tsai MH,Chuang EY,Chuang LL,Lai LC

49-1033 was used in Chromatin immunoprecipitation to reveal novel regulatory mechanisms of NDRG1-OT1 by HIF-1α and upon miR-875-3p. Also, NDRG1-OT1 promoted the malignancy of breast cancer cells and encoded a small peptide.

Tue Sep 20 00:00:00 UTC 2022

The adenoviral E1A N-terminal domain represses MYC transcription in human cancer cells by targeting both p300 and TRRAP and inhibiting MYC promoter acetylation of H3K18 and H4K16.

Genes & cancer

Zhao LJ,Loewenstein PM,Green M

49-1033 was used in ChIP assay identify host pathways targeted by viral E1A 1-80 using cancer cells

Tue Mar 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8

View more View less

Gene Aliases: 220kDa; hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2

View more View less

UniProt ID: (Human) P24928, (Mouse) P08775

View more View less

Entrez Gene ID: (Human) 5430, (Mouse) 20020

View more View less

Function(s)

RNA polymerase II activity DNA binding DNA-directed RNA polymerase activity RNA-directed RNA polymerase activity protein binding ubiquitin protein ligase binding poly(A) RNA binding metal ion binding core promoter binding transferase activity nucleotidyltransferase activity

Process(es)

transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of RNA splicing mRNA splicing, via spliceosome transcription, RNA-templated transcription-coupled nucleotide-excision repair DNA-templated transcription, termination regulation of transcription, DNA-templated transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

RNA pol II CTD Monoclonal Antibody

RNA pol II CTD Antibody (49-1033) in ChIP-seq

Product Details

49-1033

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!