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RNAse H2A Antibody (PA5-78330) in ICC/IF
RNAse H2A Peptide detects RNASEH2A protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RNASEH2A protein stained by RNAse H2A Peptide (Product # PA5-78330) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78330
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RNAse H2A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736528
Product Specific Information
Positive Control: HeLa, HeLa NE
Predicted Reactivity: Mouse (88%), Rat (85%), Pig (90%), Bovine (90%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGS4; Aicardi-Goutieres syndrome 4 protein; Ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease H2, subunit A; ribonuclease HI; Ribonuclease HI large subunit; Ribonuclease HI subunit A; ribonuclease HI, large subunit; RNase H(35); RNase H2 subunit A; RNase HI large subunit
Gene Aliases: AGS4; JUNB; RNASEH2A; RNASEHI; RNHIA; RNHL
UniProt ID: (Human) O75792
Entrez Gene ID: (Human) 10535
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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