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FIGURE: 1 / 3
RNF14 Antibody (MA5-52714) in ICC/IF
Immunocytochemistry analysis of RNF14 in HepG2 cells. Nuclei were stained blue with DAPI; RNF14 recombinant monoclonal antibody (Product # MA5-52714) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-52714
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB4610
Immunogen
A synthesized peptide derived from human RNF14 (350-375AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3249191
Target Information
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: androgen receptor associated protein 54; Androgen receptor-associated protein 54; E3 ubiquitin-protein ligase RNF14; HFB30; protein Triad2; RING finger protein 14; triad2 protein; TRIAD2 type I
Gene Aliases: 2310075C09Rik; 2610005D23Rik; AA986456; ARA54; AU041447; D18Ertd188e; D7Bwg0165e; HFB30; HRIHFB2038; RNF14; TRIAD2
UniProt ID: (Human) Q9UBS8, (Mouse) Q9JI90
Entrez Gene ID: (Human) 9604, (Rat) 619577, (Mouse) 56736
transcription coactivator activity
protein binding
zinc ion binding
ligase activity
ubiquitin-like protein transferase activity
ubiquitin conjugating enzyme binding
androgen receptor binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
protein ubiquitination
androgen receptor signaling pathway
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of transcription, DNA-templated
regulation of androgen receptor signaling pathway
metabolic process
response to estradiol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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