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RNF144A Antibody (PA5-110305) in WB
Western blot analysis of RNF144A in extracts of various cell lines. Samples were incubated with RNF144A Polyclonal antibody (Product # PA5-110305) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-110305
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 52-179 of human RNF144A (NP_055561.2).
Conjugate
Form
Liquid
Concentration
3.47 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855716
Product Specific Information
Immunogen sequence: ELLIKEGLET AISCPDAACP KQGHLQENEI ECMVAAEIMQ RYKKLQFERE VLFDPCRTWC PASTCQAVCQ LQDVGLQTPQ PVQCKACRME FCSTCKASWH PGQGCPETMP ITFLPGETSA AFKMEEDD
Target Information
UBCE7IP4 (Ubiquitin-conjugating enzyme 7- interacting protein 4), also known as RNF144A (RING finger protein 144A), KIAA0161 or RNF144, is a 292 amino acid single-pass membrane protein that contains one RING-type zinc finger and two IBR-type zinc fingers. Functioning as an E3 ubiquitin-protein ligase, UBCE7IP4 accepts ubiquitin (in the form of a thioester) from E2 ubiquitin-conjugating enzymes, such as UBC8 and UBCH7, and transfers that ubiquitin residue to target substrates. Via its RING finger, UBCE7IP4 may play a role in protein-DNA and protein-protein interactions throughout the cell.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF144A; probable E3 ubiquitin-protein ligase RNF144A; ring finger protein 144; RING finger protein 144A; UbcM4-interacting protein 4; ubiquitin conjugating enzyme 7 interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4
Gene Aliases: KIAA0161; RGD1561737; RNF144; RNF144A; UBCE7IP4
UniProt ID: (Human) P50876
Entrez Gene ID: (Human) 9781, (Rat) 500636
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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