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RNF146 Antibody (MA5-50800) in ICC/IF
Immunocytochemistry analysis of RNF146 in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with RNF146 recombinant monoclonal antibody (Product # MA5-50800) with a dilution of 1:100 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of t... View More
Product Details
MA5-50800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-29
Immunogen
Recombinant protein within full length human RNF146 aa 1-359/359.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093723
Product Specific Information
Positive control: SH-SY5Y cell lysate, NIH/3T3 cell lysate, human testis tissue, human thyroid cancer tissue, NIH/3T3 cell, SH-SY5Y cell.
Predicted band size: 39 kDa
Subcellular Location: Cytoplasm, Nucleus.
Target Information
RNF146 protein function is currently unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dactylidin; E3 ubiquitin-protein ligase RNF146; Iduna; RING finger protein 146; RING-type E3 ubiquitin transferase RNF146
Gene Aliases: 2610509H23Rik; Iduna; RNF146
UniProt ID: (Human) Q9NTX7, (Mouse) Q9CZW6
Entrez Gene ID: (Human) 81847, (Mouse) 68031
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
poly-ADP-D-ribose binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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