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FIGURE: 1 / 1
RNF181 Antibody (PA5-89806) in WB
Western blot analysis of RNF181 in extracts of various cell lines. Samples were incubated with RNF181 Polyclonal antibody (Product # PA5-89806) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89806
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_0575781)
Conjugate
Form
Liquid
Concentration
0.59 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805784
Product Specific Information
Immunogen sequence: MASYFDEHDC EPSDPEQETR TNMLLELARS LFNRMDFEDL GLVVDWDHHL PPPAAKTVVE NLPRTVIRGS QAELKCPVCL LEFEEEETAI EMPCHHLFHS SCILPWLSKT NSCPLCRYEL PTDDDTYEEH RRDKARKQQQ QHRLENLHGA MYT; Positive Samples: LO2, Mouse liver, Mouse brain, Mouse kidney, Mouse lung, Rat testis, Rat brain
Target Information
RNF181 binds the integrin alpha-IIb (ITGA2B)/beta-3 (ITGB3) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF181; RING finger protein 181
Gene Aliases: 1700047F16Rik; 2500002L14Rik; 6230415N01Rik; C77350; HSPC238; RNF181
UniProt ID: (Human) Q9P0P0, (Rat) Q6AXU4, (Mouse) Q9CY62
Entrez Gene ID: (Human) 51255, (Rat) 297337, (Mouse) 66510
ubiquitin-protein transferase activity
zinc ion binding
ligase activity
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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