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RNF2 Antibody (A302-869A) in IHC (P)
Detection of human RNF2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 (Product # A302-869A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: Vector ImmPACT NovaRED.
Product Details
A302-869A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 150 and 200 of Human Ring Finger Protein 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat and Orangutan
Target Information
RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAP 1; DinG protein; E3 ubiquitin-protein ligase RING2; GS1-120K12.1; HIP2-interacting protein 3; HIPI 3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; Protein DinG; RING 1B; RING 2; RING finger protein 1B; RING finger protein 2; RING finger protein BAP-1; RING-type E3 ubiquitin transferase RING2; RING1b; RNF 2
Gene Aliases: AI326319; AI450156; AU019207; BAP-1; BAP1; DING; HIPI3; RING1B; RING2; RNF2
UniProt ID: (Human) Q99496, (Mouse) Q9CQJ4
Entrez Gene ID: (Human) 6045, (Mouse) 19821
chromatin binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
RING-like zinc finger domain binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
gastrulation with mouth forming second
transcription, DNA-templated
germ cell development
anterior/posterior axis specification
protein sumoylation
spinal cord development
response to retinoic acid
histone H2A monoubiquitination
histone H2A-K119 monoubiquitination
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of transcription, DNA-templated
histone ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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