Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
RNF2 Recombinant Rabbit Monoclonal Antibody (13H1L14)
FIGURE: 1 / 4
RNF2 Antibody (703252) in ICC/IF
For immunofluorescence analysis, HCT 116 cells were fixed and permeabilized for detection of endogenous RNF2 using Anti-RNF2 Recombinant Rabbit Monoclonal Antibody (Product # 703252, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RNF2 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Produc... View More
Product Details
703252
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
13H1L14
Immunogen
Peptides corresponding to human RNF2 [1) aa183-aa201 2) aa155-aa174]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2762381
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Rat, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: BAP 1; DinG protein; E3 ubiquitin-protein ligase RING2; GS1-120K12.1; HIP2-interacting protein 3; HIPI 3; Huntingtin-interacting protein 2-interacting protein 3; OTTHUMP00000060668; Protein DinG; RING 1B; RING 2; RING finger protein 1B; RING finger protein 2; RING finger protein BAP-1; RING-type E3 ubiquitin transferase RING2; RING1b; RNF 2
Gene Aliases: BAP-1; BAP1; DING; HIPI3; RING1B; RING2; RNF2
UniProt ID: (Human) Q99496
Entrez Gene ID: (Human) 6045
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
gastrulation with mouth forming second
transcription, DNA-templated
germ cell development
anterior/posterior axis specification
protein sumoylation
spinal cord development
response to retinoic acid
histone H2A monoubiquitination
histone H2A-K119 monoubiquitination
negative regulation of sequence-specific DNA binding transcription factor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support