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Invitrogen
RNF220 Polyclonal Antibody
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FIGURE: 1 / 1
RNF220 Antibody (PA5-110308) in WB
Western blot analysis of RNF220 in various lysates. Samples were incubated with RNF220 Polyclonal antibody (Product # PA5-110308) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-110308
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 267-566 of human RNF220 (NP_060620.2).
Conjugate
Form
Liquid
Concentration
1.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855719
Product Specific Information
Immunogen sequence: PKSLLLSASI KREGESPTAS PHSSATDDLH HSDRYQTFLR VRANRQTRLN ARIGKMKRRK QDEGQREGSC MAEDDAVDIE HENNNRFEEY EWCGQKRIRA TTLLEGGFRG SGFIMCSGKE NPDSDADLDV DGDDTLEYGK PQYTEADVIP CTGEEPGEAK EREALRGAVL NGGPPSTRIT PEFSKWASDE MPSTSNGESS KQEAMQKTCK NSDIEKITED SAVTTFEALK ARVRELERQL SRGDRYKCLI CMDSYSMPLT SIQCWHVHCE ECWLRTLGAK KLCPQCNTIT APGDLRRIYL
Target Information
E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B. Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling (PubMed:25266658).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF220; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220
Gene Aliases: 4732477A13; 4931406I20Rik; 5730503K05Rik; AV302541; C1orf164; RNF220
UniProt ID: (Human) Q5VTB9, (Mouse) Q6PDX6
Entrez Gene ID: (Human) 55182, (Rat) 500532, (Mouse) 66743
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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