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Rockland
RNF25 Polyclonal Antibody
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FIGURE: 1 / 2
RNF25 Antibody (600-401-969) in WB
Western blot using Rocklands affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 1 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
Product Details
600-401-969
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human RNF25 protein. The antibody also recognizes mouse RNF25 protein. Reactivity against homologues from other sources has not been determined.
Target Information
The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF25; RING finger protein 25; RING finger protein AO7
Gene Aliases: 0610009H16Rik; AO7; RNF25
UniProt ID: (Human) Q96BH1, (Mouse) Q9QZR0
Entrez Gene ID: (Human) 64320, (Mouse) 57751
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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