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RNF41 Antibody (CF811158) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF41 (RC212145, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF41. Positive lysates LY430682 (100 µg) and LC430682 (20 µg) can be purchased separately from OriGene.
Product Details
CF811158
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2C1
Immunogen
Full length human recombinant protein of human RNF41 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. Nrdp1, also known as RNF41 (RING finger protein 41), SBBI03 or FLRF, is a 317 amino acid protein that contains one RING-type zinc finger and one SIAH-type zinc finger. Expressed in testis, ovary and prostate, Nrdp1 functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Specifically, Nrdp1 interacts with ErbB-3 and UBPY, thereby targeting them for proteasomal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase NRDP1; fetal liver ring finger; neuregulin receptor degradation protein-1; RING finger protein 41; ring finger protein 41, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase NRDP1
Gene Aliases: 2210404G21Rik; 4930511A05Rik; 4933415P08Rik; D10Ertd722e; FLRF; NRDP1; RNF41; SBBI03
UniProt ID: (Human) Q9H4P4, (Mouse) Q8BH75
Entrez Gene ID: (Human) 10193, (Mouse) 67588, (Rat) 362814
ubiquitin-protein transferase activity
erythropoietin receptor binding
interleukin-3 receptor binding
protein binding
zinc ion binding
acid-amino acid ligase activity
Ral GTPase binding
protein tag
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
autophagy
negative regulation of cell proliferation
proteasomal protein catabolic process
negative regulation of cell migration
ERBB2 signaling pathway
regulation of MAPK cascade
regulation of lymphocyte differentiation
regulation of myeloid cell differentiation
positive regulation of sequence-specific DNA binding transcription factor activity
protein autoubiquitination
regulation of protein kinase B signaling
extrinsic apoptotic signaling pathway
regulation of establishment of cell polarity
regulation of reactive oxygen species metabolic process
positive regulation of reactive oxygen species metabolic process
apoptotic process
regulation of gene expression
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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