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Invitrogen
RNF5 Polyclonal Antibody
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FIGURE: 1 / 4
RNF5 Antibody (PA5-103822) in ICC/IF
Immunocytochemistry analysis of RNF5 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RNF5 polyclonal antibody (Product # PA5-103822) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103822
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RNF5(Accession Q99942), corresponding to amino acid residues F131-I180.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853155
Product Specific Information
Antibody detects endogenous levels of total RNF5.
Target Information
The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF5; HsRma1; ing finger protein 5 pseudogene; protein G16; Ram1 homolog; RING finger protein 5; ring finger protein 5, E3 ubiquitin protein ligase
Gene Aliases: 2410131O05Rik; AA407576; G16; NG2; RING5; RMA1; RNF5
UniProt ID: (Human) Q99942, (Mouse) O35445, (Rat) Q5M807
Entrez Gene ID: (Human) 6048, (Mouse) 54197, (Rat) 407784
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity involved in ERAD pathway
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
response to bacterium
negative regulation of autophagy
protein destabilization
ERAD pathway
cellular protein catabolic process
protein K63-linked ubiquitination
protein K48-linked ubiquitination
ER-associated misfolded protein catabolic process
regulation of autophagosome assembly
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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