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Invitrogen
ROCK1 Monoclonal Antibody (1H4)
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Product Details
MA5-17165
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1H4
Immunogen
Purified recombinant fragment of human ROCK1 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538636
Product Specific Information
MA5-17165 targets ROCK1 in indirect ELISA and WB applications and shows reactivity with Human samples.
The MA5-17165 immunogen is purified recombinant fragment of human ROCK1 expressed in E. Coli.
MA5-17165 detects ROCK1 which has a predicted molecular weight of approximately 158kDa.
Target Information
ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC131603; MGC43611; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I
Gene Aliases: P160ROCK; ROCK-I; ROCK1
UniProt ID: (Human) Q13464
Entrez Gene ID: (Human) 6093
apical constriction
protein phosphorylation
cellular component disassembly involved in execution phase of apoptosis
smooth muscle contraction
leukocyte cell-cell adhesion
signal transduction
I-kappaB kinase/NF-kappaB signaling
Rho protein signal transduction
negative regulation of angiogenesis
membrane to membrane docking
actin cytoskeleton organization
regulation of cell adhesion
cortical actin cytoskeleton organization
bleb assembly
negative regulation of protein binding
regulation of actin cytoskeleton organization
negative regulation of myosin-light-chain-phosphatase activity
negative regulation of neuron apoptotic process
regulation of keratinocyte differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
leukocyte migration
leukocyte tethering or rolling
myoblast migration
regulation of stress fiber assembly
regulation of focal adhesion assembly
positive regulation of focal adhesion assembly
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
regulation of establishment of cell polarity
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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