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ROCK2(middle) Polyclonal Antibody

ROCK2(middle) Antibody in Immunocytochemistry (ICC/IF)

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ROCK2(middle) Antibody (21645-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using (Product # 21645-1-AP) (ROCK2(middle) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ROCK2(middle) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ROCK2(middle) Antibody in Western Blot (WB)

ROCK2(middle) Antibody in Immunoprecipitation (IP)

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Product Details

21645-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ROCK2(middle) Fusion Protein Ag16330 (1-181 aa encoded by BC111801)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.52 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to ROCK2.

Immunogen sequence: MEIDMTYQL KVIQQSLEQE EAEHKATKAR LADKNKIYES IEEAKSEAMK EMEKKLLEER TLKQKVENLL LEAEKRCSLL DCDLKQSQQK INELLKQKDV LNEDVRNLTL KIEQETQKRC LTQNDLKMQT QQVNTLKMSE KQLKQENNHL MEMKMNLEKQ NAELRKERQD ADGQMKELQD QL (1-181 aa encoded by BC111801)

Target Information

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway.

International journal of molecular medicine

Yang J,Wang L,Zhang Z,Sun Q,Zhang Y

Sat Jul 01 00:00:00 UTC 2023

Diosmetin alleviates acute lung injury caused by lipopolysaccharide by targeting barrier function.

Inflammopharmacology

Xia J,Li J,Deng M,Yin F,Liu J,Wang J

Tue Aug 01 00:00:00 UTC 2023

Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis.

Bioengineered

Chong S,Chen G,Dang Z,Niu F,Zhang L,Ma H,Zhao Y

Fri Apr 01 00:00:00 UTC 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 UTC 2022

Oxycodone relieves permeability damage and apoptosis of oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cells through ras homolog family member A (RhoA)/ Rho-associated coiled-coil containing kinases (ROCK)/ myosin light chain 2 (MLC2) signal.

Bioengineered

Shao F,Han D,Shen Y,Bian W,Zou L,Hu Y,Sun W

Tue Mar 01 00:00:00 UTC 2022

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

Cells

Becker KN,Pettee KM,Sugrue A,Reinard KA,Schroeder JL,Eisenmann KM

Thu May 05 00:00:00 UTC 2022

Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.

Frontiers in pharmacology

Yuan P,Ma D,Gao X,Wang J,Li R,Liu Z,Wang T,Wang S,Liu J,Liu X

Sat Apr 16 00:00:00 UTC 2022

JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.

Andrology

Liu K,Cui K,Feng H,Li R,Lin H,Chen Y,Zhang Y,Chen Z,Yuan H,Li M,Wang T,Lan R,Liu J,Rao K,Wen B

Sun Mar 01 00:00:00 UTC 2020

JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.

Andrology

Liu K,Cui K,Feng H,Li R,Lin H,Chen Y,Zhang Y,Chen Z,Yuan H,Li M,Wang T,Lan R,Liu J,Rao K,Wen B

Sun Mar 01 00:00:00 UTC 2020

Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.

Molecular pharmacology

Wang Y,Jiang L,Mo X,Lan Y,Yang X,Liu X,Zhang J,Zhu L,Liu J,Wu X

Fri Sep 01 00:00:00 UTC 2017

Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.

Molecular pharmacology

Wang Y,Jiang L,Mo X,Lan Y,Yang X,Liu X,Zhang J,Zhu L,Liu J,Wu X

Fri Sep 01 00:00:00 UTC 2017

FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

The journal of sexual medicine

Cui K,Ruan Y,Wang T,Rao K,Chen Z,Wang S,Liu J

Wed Mar 01 00:00:00 UTC 2017

FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

The journal of sexual medicine

Cui K,Ruan Y,Wang T,Rao K,Chen Z,Wang S,Liu J

Wed Mar 01 00:00:00 UTC 2017

Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents.

ACS nano

Zhu J,Xu M,Gao M,Zhang Z,Xu Y,Xia T,Liu S

Tue Mar 28 00:00:00 UTC 2017

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Oncotarget

Ching RH,Lau EY,Ling PM,Lee JM,Ma MK,Cheng BY,Lo RC,Ng IO,Lee TK

Tue Dec 22 00:00:00 UTC 2015

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Oncotarget

Ching RH,Lau EY,Ling PM,Lee JM,Ma MK,Cheng BY,Lo RC,Ng IO,Lee TK

Tue Dec 22 00:00:00 UTC 2015

Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.

Biochemical and biophysical research communications

Huang D,Du X,Yuan R,Chen L,Liu T,Wen C,Huang M,Li M,Hao L,Shao J

Fri Oct 10 00:00:00 UTC 2014

Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.

Biochemical and biophysical research communications

Huang D,Du X,Yuan R,Chen L,Liu T,Wen C,Huang M,Li M,Hao L,Shao J

Fri Oct 10 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha

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Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha

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UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336

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Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878

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Function(s)

protein serine/threonine kinase activity structural molecule activity protein binding ATP binding Rho GTPase binding poly(A) RNA binding metal ion binding Rho-dependent protein serine/threonine kinase activity kinase activity nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

neural tube closure protein phosphorylation smooth muscle contraction Rho protein signal transduction extrinsic apoptotic signaling pathway via death domain receptors positive regulation of centrosome duplication negative regulation of angiogenesis actin cytoskeleton organization chromosome condensation regulation of actin cytoskeleton organization regulation of circadian rhythm regulation of keratinocyte differentiation rhythmic process dendrite morphogenesis centrosome duplication phosphorylation intracellular signal transduction cytokinesis positive regulation of protein phosphorylation I-kappaB kinase/NF-kappaB signaling positive regulation of endothelial cell migration positive regulation of gene expression regulation of cell adhesion cortical actin cytoskeleton organization negative regulation of myosin-light-chain-phosphatase activity vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to testosterone stimulus establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly regulation of establishment of cell polarity regulation of cell motility

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ROCK2(middle) Polyclonal Antibody

ROCK2(middle) Antibody (21645-1-AP) in ICC/IF

Product Details

21645-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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