Proteintech

RPB5 Polyclonal Antibody

RPB5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RPB5 Antibody (15217-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15217-1-AP (RPB5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

RPB5 Antibody in Immunoprecipitation (IP)

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Product Details

15217-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPB5 Fusion Protein Ag7386 (1-210 aa encoded by BC004441)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDDEEETYR LWKIRKTIMQ LCHDRGYLVT QDELDQTLEE FKAQFGDKPS EGRPRRTDLT VLVAHNDDPT DQMFVFFPEE PKVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL LARYKLRENQ LPRIQAGDPV ARYFGIKRGQ VVKIIRPSET AGRYITYRLV Q (1-210 aa encoded by BC004441)

Target Information

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.

Molecular cell

Li Y,Huang J,Bao L,Zhu J,Duan W,Zheng H,Wang H,Jiang Y,Liu W,Zhang M,Yu Y,Yi C,Ji X

Thu Apr 20 00:00:00 UTC 2023

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture.

Genome biology

Jiang Y,Huang J,Tian K,Yi X,Zheng H,Zhu Y,Guo T,Ji X

Mon Nov 28 00:00:00 UTC 2022

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity.

Molecular cell

Li Y,Huang J,Zhu J,Bao L,Wang H,Jiang Y,Tian K,Wang R,Zheng H,Duan W,Lai W,Yi X,Zhu Y,Guo T,Ji X

Thu Oct 20 00:00:00 UTC 2022

SPT5 stabilization of promoter-proximal RNA polymerase II.

Molecular cell

Aoi Y,Takahashi YH,Shah AP,Iwanaszko M,Rendleman EJ,Khan NH,Cho BK,Goo YA,Ganesan S,Kelleher NL,Shilatifard A

Thu Nov 04 00:00:00 UTC 2021

RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.

Oncogene

Zhang J,Pan YF,Ding ZW,Yang GZ,Tan YX,Yang C,Jiang TY,Liu LJ,Zhang B,Han T,Cao D,Yang T,Yang N,Wu MC,Dong LW,Wang HY

Thu Mar 19 00:00:00 UTC 2015

RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.

Oncogene

Zhang J,Pan YF,Ding ZW,Yang GZ,Tan YX,Yang C,Jiang TY,Liu LJ,Zhang B,Han T,Cao D,Yang T,Yang N,Wu MC,Dong LW,Wang HY

Thu Mar 19 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerase subunit RPABC1; DNA-directed RNA polymerases I, II, and III subunit RPABC1; polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; polymerase (RNA) II subunit E; RNA polymerases I, II, and III subunit ABC1; RPB5 homolog; XAP4

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Gene Aliases: 2410021N14Rik; 25kDa; AW208866; hRPB25; hsRPB5; POLR2E; RPABC1; RPB5; XAP4

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UniProt ID: (Human) P19388, (Mouse) Q80UW8, (Rat) B0BNE2

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Entrez Gene ID: (Human) 5434, (Mouse) 66420, (Rat) 690966

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Function(s)

RNA polymerase I activity RNA polymerase II activity RNA polymerase III activity DNA binding DNA-directed RNA polymerase activity protein binding molecular_function

Process(es)

transcription, DNA-templated transcription from RNA polymerase II promoter mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription from RNA polymerase III promoter fibroblast growth factor receptor signaling pathway gene expression viral process gene silencing by RNA positive regulation of type I interferon production somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of gene expression, epigenetic positive regulation of viral transcription transcription from RNA polymerase I promoter biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPB5 Polyclonal Antibody

RPB5 Antibody (15217-1-AP) in IHC (P)

Product Details

15217-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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