Invitrogen

RPL11 Monoclonal Antibody (3A4A7)

RPL11 Antibody in Immunocytochemistry (ICC/IF)

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RPL11 Antibody (37-3000) in ICC/IF

Immunofluorescence analysis of L11 was performed using 70% confluent log phase U-2 OS cells treated with doxorubicin (2 µg/mL) for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RPL11 (3A4A7) Mouse Monoclonal Antibody (Product # 37-3000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Anti... View More

RPL11 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

RPL11 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of RPL11 using RPL11 monoclonal antibody (Product # 37-3000), shows induction of RPL11 expression in the nucleus of U2OS cells upon doxorubicin treatment. {TM}

Product Details

37-3000

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A4A7

Immunogen

fusing the mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with human Laminin (1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533310

Target Information

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Adaptive translational reprogramming of metabolism limits the response to targeted therapy in BRAF^V600 melanoma.

Nature communications

Smith LK,Parmenter T,Kleinschmidt M,Kusnadi EP,Kang J,Martin CA,Lau P,Patel R,Lorent J,Papadopoli D,Trigos A,Ward T,Rao AD,Lelliott EJ,Sheppard KE,Goode D,Hicks RJ,Tiganis T,Simpson KJ,Larsson O,Blythe B,Cullinane C,Wickramasinghe VO,Pearson RB,McArthur GA

37-3000 was used in Western Blot to propose selective mRNA processing and translation by UHMK1 constitutes a mechanism of non-genetic resistance to targeted therapy in melanoma by controlling metabolic plasticity induced by therapy.

Tue Mar 01 00:00:00 UTC 2022

The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output.

Science advances

Kanellis DC,Espinoza JA,Zisi A,Sakkas E,Bartkova J,Katsori AM,Boström J,Dyrskjøt L,Broholm H,Altun M,Elsässer SJ,Lindström MS,Bartek J

37-3000 was used in Western Blot to unravel eIF4A3's tumor-promoting function by demonstrating its role in ribosome biogenesis (RiBi) and p53 (de)regulation.

Sun Aug 01 00:00:00 UTC 2021

Ribosomal Protein L15 is involved in Colon Carcinogenesis.

International journal of medical sciences

Dong Z,Jiang H,Liang S,Wang Y,Jiang W,Zhu C

Published figure using RPL11 monoclonal antibody (Product # 37-3000) in Immunofluorescence

Fri Jan 31 00:00:00 UTC 2020

Ribosomal Protein L15 is involved in Colon Carcinogenesis.

International journal of medical sciences

Dong Z,Jiang H,Liang S,Wang Y,Jiang W,Zhu C

Published figure using RPL11 monoclonal antibody (Product # 37-3000) in Immunofluorescence

Fri Jan 31 00:00:00 UTC 2020

Direct relationship between the level of p53 stabilization induced by rRNA synthesis-inhibiting drugs and the cell ribosome biogenesis rate.

Oncogene

Scala F,Brighenti E,Govoni M,Imbrogno E,Fornari F,Treré D,Montanaro L,Derenzini M

37-3000 was used in western blot to study the role of p53 stabilization in the effects of chemotherapy drugs

Thu Feb 25 00:00:00 UTC 2016

Acrolein preferentially damages nucleolus eliciting ribosomal stress and apoptosis in human cancer cells.

Oncotarget

Wang HT,Chen TY,Weng CW,Yang CH,Tang MS

37-3000 was used in Western Blotting to examine the mechanisms by which acrolein exerts its anti-cancer activity and cytotoxicity.

Tue Dec 06 00:00:00 UTC 2016

p53 is positively regulated by miR-542-3p.

Cancer research

Wang Y,Huang JW,Castella M,Huntsman DG,Taniguchi T

Published figure using RPL11 monoclonal antibody (Product # 37-3000) in Western Blot

Sun Jun 15 00:00:00 UTC 2014

mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels.

Cancer biology & therapy

Goudarzi KM,Nistér M,Lindström MS

37-3000 was used in Western Blotting to investigate how the p53 response to nucleolar stress is affected by simultaneous mTOR inhibition in osteosarcoma and glioma cell lines.

Fri Aug 07 00:00:00 UTC 2015

mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels.

Cancer biology & therapy

Goudarzi KM,Nistér M,Lindström MS

37-3000 was used in Western Blotting to investigate how the p53 response to nucleolar stress is affected by simultaneous mTOR inhibition in osteosarcoma and glioma cell lines.

Fri Aug 07 00:00:00 UTC 2015

Growth inhibitory effects of large subunit ribosomal proteins in melanoma.

Pigment cell & melanoma research

Kardos GR,Dai MS,Robertson GP

37-3000 was used in western blot to evaluate large subunit ribosomal proteins as therapeutic targets for melanoma

Mon Sep 01 00:00:00 UTC 2014

5S ribosomal RNA is an essential component of a nascent ribosomal precursor complex that regulates the Hdm2-p53 checkpoint.

Cell reports

Donati G,Peddigari S,Mercer CA,Thomas G

37-3000 was used in Western Blotting to study the effects of small interfering RNAs directed against 5S ribosomal RNA on the nascent levels of noncoding ribosomal RNA, and the Hdm4 inhibition of p53.

Thu Jul 11 00:00:00 UTC 2013

Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus.

Journal of virology

Chen W,Dittmer DP

37-3000 was used in Immunoprecipitation to show that ribosomal protein S6 interacts with LANA.

Thu Sep 01 00:00:00 UTC 2011

Ribosomal 18 S RNA processing by the IGF-I-responsive WDR3 protein is integrated with p53 function in cancer cell proliferation.

The Journal of biological chemistry

McMahon M,Ayllón V,Panov KI,O'Connor R

Fri Jun 11 00:00:00 UTC 2010

Ribosomal 18 S RNA processing by the IGF-I-responsive WDR3 protein is integrated with p53 function in cancer cell proliferation.

The Journal of biological chemistry

McMahon M,Ayllón V,Panov KI,O'Connor R

Fri Jun 11 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: 60S ribosomal protein L11; cell growth-inhibiting protein 34; CLL-associated antigen KW-12; Large ribosomal subunit protein uL5; RP11-223J15.3

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Gene Aliases: DBA7; GIG34; L11; RPL11

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UniProt ID: (Human) P62913

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Entrez Gene ID: (Human) 6135

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Function(s)

RNA binding structural constituent of ribosome protein binding rRNA binding poly(A) RNA binding

Process(es)

ribosomal large subunit assembly nuclear-transcribed mRNA catabolic process, nonsense-mediated decay rRNA processing translation translational initiation protein targeting SRP-dependent cotranslational protein targeting to membrane viral transcription protein localization to nucleus ribosomal large subunit biogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPL11 Monoclonal Antibody (3A4A7)

RPL11 Antibody (37-3000) in ICC/IF

Product Details

37-3000

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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