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Invitrogen
RPL29 Polyclonal Antibody
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FIGURE: 1 / 4
RPL29 Antibody (PA5-103543) in ICC/IF
Immunocytochemistry analysis of RPL29 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPL29 polyclonal antibody (Product # PA5-103543) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103543
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RPL29(Accession P47914), corresponding to amino acid residues K22-I72.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852879
Product Specific Information
Antibody detects endogenous levels of total RPL29.
Target Information
Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L29; Cell surface heparin-binding protein HIP; heparin/heparan sulfate-binding protein; heparin/heparan sulfate-interacting protein; HP/HS-interacting protein; Large ribosomal subunit protein eL29; P23; ribosomal protein YL43 homologue
Gene Aliases: HIP; HUMRPL29; L29; RPL29; RPL29_3_370; RPL29P10; Rpl43
UniProt ID: (Human) P47914, (Rat) P25886, (Mouse) P47915
Entrez Gene ID: (Human) 6159, (Rat) 29283, (Mouse) 19944
translation
cell proliferation
cell-substrate adhesion
multicellular organism growth
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cytoplasmic translation
rRNA processing
translational initiation
SRP-dependent cotranslational protein targeting to membrane
embryo implantation
viral transcription
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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