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RPL35A Antibody (PA5-110319) in WB
Western blot analysis of RPL35A in extracts of mouse stomach. Samples were incubated with RPL35A Polyclonal antibody (Product # PA5-110319) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Product Details
PA5-110319
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 10-90 of human RPL35A (NP_000987.2).
Conjugate
Form
Liquid
Concentration
1.21 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855730
Product Specific Information
Immunogen sequence: IFAGYKRGLR NQREHTALLK IEGVYARDET EFYLGKRCAY VYKAKNNTVT PGGKPNKTRV IWGKVTRAHG NSGMVRAKFR S
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L35a; Large ribosomal subunit protein eL33
Gene Aliases: 2810431L15Rik; Rpl35; Rpl35a
UniProt ID: (Rat) P04646, (Mouse) O55142
Entrez Gene ID: (Rat) 57809, (Mouse) 57808
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