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FIGURE: 1 / 1
RPL7A Antibody (MA5-37881) in WB
Western blot analysis of RPL7A in extracts of various cell lines. Samples were incubated with RPL7A Monoclonal antibody (Product # MA5-37881) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
MA5-37881
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4I8Y6
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPL7A (P62424)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897801
Product Specific Information
Positive Samples: 293T, PC-12, HepG2, NIH/3T3, Raji, Mouse lung, Mouse liver, Rat lung, Rat liver
Immunogen sequence: MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDRQ TATQLLKLAH KYRPETKQEK KQRLLARAEK KAAGKGDVPT KRPPVLRAGV NTVTTLVENK KAQLVVIAHD VDPIE
Target Information
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L7a; Large ribosomal subunit protein eL8; PLA-X polypeptide; surfeit 3; Surfeit locus protein 3; thyroid hormone receptor uncoupling protein
Gene Aliases: L7A; RPL7A; SURF-3; SURF3; TRUP
UniProt ID: (Human) P62424, (Mouse) P12970, (Rat) P62425
Entrez Gene ID: (Human) 6130, (Mouse) 27176, (Rat) 296596
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