Proteintech

RPN1 Polyclonal Antibody

RPN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

RPN1 Antibody (12894-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

12894-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPN1 Fusion Protein Ag3576 (22-350 aa encoded by BC010839)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GSASSEAPP LINEDVKRTV DLSSHLAKVT AEVVLAHLGG GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV RETKIKGKSG RFFTVKLPVA LDPGAKISVI VETVYTHVLH PYPTQITQSE KQFVVFEGNH YFYSPYPTKT QTMRVKLASR NVESYTKLGN PTRSEDLLDY GPFRDVPAYS QDTFKVHYEN NSPFLTITSM TRVIEVSHWG NIAVEENVDL KHTGAVLKGP FSRYDYQRQP DSGISSIRSF KTILPAAAQD VYYRDEIGNV STSHLLILDD SVEMEIRPRF PLFGGWKTHY IVGYNLPSYE YLYNLGDQYA (22-350 aa encoded by BC010839)

Target Information

Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

[WITHDRAWN] Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction.

Research square

Tatekoshi Y,Shapiro JS,Liu M,Bianco GM,Tatekoshi A,De Jesus A,Koleini N,Wasserstrom JA,Dillmann WH,Weinberg SE,Ardehali H

Wed Jan 25 00:00:00 UTC 2023

Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation.

Science advances

Phoomak C,Rinis N,Baro M,Shrimal S,Bennett D,Shaffer SA,Lehrman M,Gilmore R,Contessa JN

Fri Mar 17 00:00:00 UTC 2023

Phenylbutyrate rescues the transport defect of the Sec61α mutations V67G and T185A for renin.

Life science alliance

Sicking M,Živná M,Bhadra P,Barešová V,Tirincsi A,Hadzibeganovic D,Hodaňová K,Vyleťal P,Sovová J,Jedličková I,Jung M,Bell T,Helms V,Bleyer AJ,Kmoch S,Cavalié A,Lang S

Fri Apr 01 00:00:00 UTC 2022

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Science advances

Phoomak C,Cui W,Hayman TJ,Yu SH,Zhao P,Wells L,Steet R,Contessa JN

Fri Jan 01 00:00:00 UTC 2021

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

International journal of molecular sciences

Zhang W,Huang B,Gao L,Huang C

Wed Apr 21 00:00:00 UTC 2021

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Proteomics

Wang Y,Park D,Galermo AG,Gao D,Liu H,Lebrilla CB

Thu Dec 01 00:00:00 UTC 2016

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Proteomics

Wang Y,Park D,Galermo AG,Gao D,Liu H,Lebrilla CB

Thu Dec 01 00:00:00 UTC 2016

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Nature chemical biology

Lopez-Sambrooks C,Shrimal S,Khodier C,Flaherty DP,Rinis N,Charest JC,Gao N,Zhao P,Wells L,Lewis TA,Lehrman MA,Gilmore R,Golden JE,Contessa JN

Thu Dec 01 00:00:00 UTC 2016

Reconfiguration of the proteasome during chaperone-mediated assembly.

Nature

Park S,Li X,Kim HM,Singh CR,Tian G,Hoyt MA,Lovell S,Battaile KP,Zolkiewski M,Coffino P,Roelofs J,Cheng Y,Finley D

Thu May 23 00:00:00 UTC 2013

Reconfiguration of the proteasome during chaperone-mediated assembly.

Nature

Park S,Li X,Kim HM,Singh CR,Tian G,Hoyt MA,Lovell S,Battaile KP,Zolkiewski M,Coffino P,Roelofs J,Cheng Y,Finley D

Thu May 23 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Dolichyl-diphosphooligosaccharide--protein; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; oligosaccharyltransfe; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); Ribophorin I; Ribophorin-1; RPN-I

View more View less

Gene Aliases: AU018702; D6Wsu137e; OST1; RBPH1; RIBI; Rpn-1; RPN-I; RPN1

View more View less

UniProt ID: (Human) P04843, (Mouse) Q91YQ5

View more View less

Entrez Gene ID: (Human) 6184, (Rat) 25596, (Mouse) 103963

View more View less

Function(s)

dolichyl-diphosphooligosaccharide-protein glycotransferase activity protein binding poly(A) RNA binding transferase activity transferase activity, transferring glycosyl groups

Process(es)

protein glycosylation protein N-linked glycosylation via asparagine cellular protein modification process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

RPN1 Polyclonal Antibody

RPN1 Antibody (12894-1-AP) in IHC (P)

Product Details

12894-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!