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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RPP25 Antibodies

          Proteintech

          RPP25 Polyclonal Antibody

          1 Reference
          View all (5) RPP25 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite RPP25 Polyclonal Antibody

          • Antibody Testing Data (8)
          RPP25 Antibody in Immunocytochemistry (ICC/IF)
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          RPP25 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          RPP25 Antibody (15461-1-AP) in ICC/IF

          Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 15461-1-AP (RPP25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RPP25 Antibody in Immunocytochemistry (ICC/IF)
          RPP25 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPP25 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPP25 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPP25 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPP25 Antibody in Western Blot (WB)
          RPP25 Antibody in Western Blot (WB)
          RPP25 Antibody in Immunoprecipitation (IP)

          Product Details

          15461-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:1,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          RPP25 Fusion Protein Ag7742 (1-199 aa encoded by BC002497)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MENFRKVRS EEAPAGCGAE GGGPGSGPFA DLAPGAVHMR VKEGSKIRNL MAFATASMAQ PATRAIVFSG CGRATTKTVT CAEILKRRLA GLHQVTRLRY RSVREVWQSL PPGPTQGQTP GEPAASLSVL KNVPGLAILL SKDALDPRQP GYQPPNPHPG PSSPPAAPAS KRSLGEPAAG EGSAKRSQPE PGVADEDQTA (1-199 aa encoded by BC002497)

          Target Information

          Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:12003489, PubMed:16723659, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465). [UniProt]

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ribonuclease P 25 subunit; ribonuclease P 25kDa subunit; Ribonuclease P protein subunit p25; ribonuclease P/MRP 25kDa subunit; RNase P protein subunit p25

          View more View less

          Gene Aliases: AI851155; RPP25

          View more View less

          UniProt ID: (Human) Q9BUL9, (Rat) Q5PPN2, (Mouse) Q91WE3

          View more View less

          Entrez Gene ID: (Human) 54913, (Rat) 315705, (Mouse) 102614

          View more View less

          Function(s)
          ribonuclease P activity protein binding poly(A) RNA binding molecular_function nucleic acid binding RNA binding hydrolase activity
          Process(es)
          tRNA processing biological_process RNA phosphodiester bond hydrolysis, endonucleolytic tRNA 5'-leader removal rRNA processing
          It has to be done as per old AB suggested Products section.
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