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RPRD1B Antibody (PA5-148238) in ICC/IF
Immunocytochemistry analysis of RPRD1B in Hela cells. Samples were incubated with RPRD1B polyclonal antibody (Product # PA5-148238) using a dilution of 1:100 at 4° overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Positive staining was localized to Nucleus.
Product Details
PA5-148238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human RPRD1B Protein (Met1-Asp326)
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3247407
Target Information
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; Regulation of nuclear pre-mRNA domain-containing protein 1B
Gene Aliases: C20orf77; CREPT; dJ1057B20.2; NET60; RPRD1B
UniProt ID: (Human) Q9NQG5
Entrez Gene ID: (Human) 58490
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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