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FIGURE: 1 / 1
RPRD1B Antibody (PA5-87631) in WB
Western blot analysis of RPRD1B in extracts of various cell lines. Samples were incubated with RPRD1B Polyclonal antibody (Product # PA5-87631) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-87631
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-326 of human rprd1b (Q9NQG5)
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804302
Product Specific Information
Positive Samples: MCF7, SKOV3, NCI-H460, Mouse liver, Mouse lung, Mouse stomach, Mouse spleen, Rat liver; Cellular Location: Nucleus
Immunogen sequence: EFESVLVDAF SHVAREADEG CKKPLERLLN IWQERSVYGG EFIQQLKLSM EDSKSPPPKA TEEKKSLKRT FQQIQEEEDD DYPGSYSPQD PSAGPLLTEE LIKALQDLEN AASGDATVRQ KIASLPQEVQ DVSLLEKITD KEAAERLSKT VDEACLLLAE YNGRLAAELE DRRQLARMLV EYTQNQKDVL SEKEKKLEEY KQKLARVTQV RKELKSHIQS LPDLSLLPNV TGGLAPLPSA GDLFSTD
Target Information
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; Regulation of nuclear pre-mRNA domain-containing protein 1B
Gene Aliases: 2610304G08Rik; 2810446G03Rik; C20orf77; CREPT; dJ1057B20.2; NET60; RGD1304782; RPRD1B
UniProt ID: (Human) Q9NQG5, (Mouse) Q9CSU0
Entrez Gene ID: (Human) 58490, (Rat) 311591, (Mouse) 70470
positive regulation of cell proliferation
regulation of cell cycle process
snRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
dephosphorylation of RNA polymerase II C-terminal domain
transcription, DNA-templated
regulation of transcription, DNA-templated
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