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RPS21 Antibody (PA5-103534) in ICC/IF
Immunocytochemistry analysis of RPS21 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RPS21 polyclonal antibody (Product # PA5-103534) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103534
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RPS21(Accession P63220), corresponding to amino acid residues V39-F83.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852870
Product Specific Information
Antibody detects endogenous levels of total RPS21.
Target Information
Differentiated embryo-chondrocyte expressed gene 1 (DEC1) is an important protein involved in embryonic cell differentiation, proliferation, and apoptosis. It has also recently been shown to be regulated by hypoxia. The loss of DEC1 expression may be an early event in the development of lung cancer, while DEC1 may be up-regulated by hypoxia in other cancers and in more extreme hypoxia it may have a role in cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40S ribosomal protein S21; 8.2 kDa differentiation factor; human leukemia differentiation factor; Small ribosomal subunit protein eS21
Gene Aliases: 1810049N11Rik; 2410030A14Rik; HLDF; RPS21; S21
UniProt ID: (Human) P63220, (Mouse) Q9CQR2, (Rat) P05765
Entrez Gene ID: (Human) 6227, (Mouse) 66481, (Rat) 81775
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
translation
ribosomal small subunit biogenesis
translational elongation
biological_process
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
rRNA processing
translational initiation
SRP-dependent cotranslational protein targeting to membrane
viral transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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