Invitrogen

RPSA Monoclonal Antibody (MLuC5)

1 Published Figure

10 References

View all (31) RPSA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (1)

RPSA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

RPSA Antibody (MA5-12840) in ICC/IF

Immunofluorescent analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Laminin Receptor monoclonal antibody (Product # MA5-12840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated... View More

RPSA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-12840

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:200

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

Immunocytochemistry (ICC/IF)

1:10-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Neutralization (Neu)

View 3 publications 3 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

MLuC5

Immunogen

Small cell lung carcinoma N592 live cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983211

Product Specific Information

MA5-12840 targets Laminin Receptor in FACS, WB, ICC/IF, and IHC (P) applications and shows reactivity with Human samples.

The MA5-12840 immunogen is small cell lung carcinoma N592 live cells.

Target Information

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The effects of cytotoxic necrotizing factor 1 expression in the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice.

Virulence

Chang AC,Krishnan S,Prasadarao NV

MA5-12840 was used in western blot to study the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice and the effects of cytotoxic necrotizing factor 1 expression

Sun Oct 02 00:00:00 UTC 2016

Inhibition of EMMPRIN and MMP-9 Expression by Epigallocatechin-3-Gallate through 67-kDa Laminin Receptor in PMA-Induced Macrophages.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang QM,Wang H,Li YF,Xie ZY,Ma Y,Yan JJ,Gao YF,Wang ZM,Wang LS

MA512840 was used in blocking or activating experiment and western blot to investigate the effect of epigallocatechin-3-gallate treatment on EMMPRIN and MMP-9 expression in PMA-induced macrophages

Mon Feb 06 00:00:00 UTC 2017

Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-α-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang ZM,Gao W,Wang H,Zhao D,Nie ZL,Shi JQ,Zhao S,Lu X,Wang LS,Yang ZJ

MA5-12840 was used in blocking or activating experiment to study the roles of NFkB and the 67-kD laminin receptor in the mechanism by which a green tea polyphenol inhibits TNF-alpha-induced MCP-1 production by HUVECs

Tue Apr 14 00:00:00 UTC 2015

Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence.

Experimental gerontology

Hinojosa CA,Mgbemena V,Van Roekel S,Austad SN,Miller RA,Bose S,Orihuela CJ

MA5-12840 was used in western blot to study the role of lowered lung cellular senescence in the mechanism by which enteric rapamycin reduces the susceptibility of aged mice to Streptococcus pneumoniae infection

Sat Dec 01 00:00:00 UTC 2012

Green tea polyphenol EGCG sensing motif on the 67-kDa laminin receptor.

PloS one

Fujimura Y,Sumida M,Sugihara K,Tsukamoto S,Yamada K,Tachibana H

MA5-12840 was used in western blot to study the functional domain of 67kDa laminin responsible for the anti-cancer activity of the green tea polyphenol (-)-epigallocatechin-3-O-gallate

Mon Oct 29 00:00:00 UTC 2012

Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor.

FEBS letters

Byun EH,Omura T,Yamada K,Tachibana H

MA5-12840 was used in blocking or activating experiment to study the molecular mechanism for the inhibition of peptidoglycan-dependent TLR2 signalling by a Green tea polyphenol.

Wed Mar 09 00:00:00 UTC 2011

High expression of 67-kDa laminin receptor relates to the proliferation of leukemia cells and increases expression of GM-CSF receptor.

Experimental hematology

Ando K,Miyazaki Y,Sawayama Y,Tominaga S,Matsuo E,Yamasaki R,Inoue Y,Iwanaga M,Imanishi D,Tsushima H,Fukushima T,Imaizumi Y,Taguchi J,Yoshida S,Hata T,Tomonaga M

MA5-12840 was used in flow cytometry to study the role of high level expression of the 67-kDa laminin receptor in GM-CSF receptor expression and proliferation of leukemia cells

Tue Feb 01 00:00:00 UTC 2011

Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.

Acta cytologica

Branca M,Giorgi C,Ciotti M,Santini D,Di Bonito L,Costa S,Benedetto A,Bonifacio D,Di Bonito P,Paba P,Accardi L,Mariani L,Syrjänen S,Favalli C,Syrjänen K

MA5-12840 was used in immunohistochemistry to investigate the prognostic value of the 67-kd laminin receptor in cervical cancer and its relationship with high-risk HPV types

Thu Mar 23 00:00:00 UTC 2006

Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone.

The European respiratory journal

Christie PE,Jonas M,Tsai CH,Chi EY,Henderson WR

MA5-12840 was used in immunocytochemistry to study the role of laminin on allergic airway remodelling in chronic allergic lung inflammation.

Thu Jul 01 00:00:00 UTC 2004

Human T cells express a functional ionotropic glutamate receptor GluR3, and glutamate by itself triggers integrin-mediated adhesion to laminin and fibronectin and chemotactic migration.

Journal of immunology (Baltimore, Md. : 1950)

Ganor Y,Besser M,Ben-Zakay N,Unger T,Levite M

MA5-12840 was used in blocking or activating experiment to study the role of GluR3 in the glutamate triggered adhesion and chemotactic migration of human T cells

Tue Apr 15 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: 37 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin-binding protein; Laminin receptor 1; laminin receptor 1 (67kD, ribosomal protein SA); Laminin-binding protein precursor p40; LamR; LBP/p40; LRP/LR; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; rssa; Small ribosomal subunit protein uS2

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Gene Aliases: 37LRP; 67LR; ICAS; LAMBR; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; NEM/1CHD4; p40; RPSA; SA

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UniProt ID: (Human) P08865

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Entrez Gene ID: (Human) 3921

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Function(s)

virus receptor activity structural constituent of ribosome laminin receptor activity protein binding ribosome binding laminin binding poly(A) RNA binding

Process(es)

ribosomal small subunit assembly nuclear-transcribed mRNA catabolic process, nonsense-mediated decay endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing rRNA export from nucleus translation translational initiation SRP-dependent cotranslational protein targeting to membrane cell adhesion viral transcription viral entry into host cell

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPSA Monoclonal Antibody (MLuC5)

RPSA Antibody (MA5-12840) in ICC/IF

Product Details

MA5-12840

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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