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RRAD Antibody (PA5-37885) in WB
Western blot analysis of RRAD using RRAD Polyclonal Antibody (Product # PA5-37885) (0.5 µg/mL) in staining of Lane A: HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 µg protein in RIPA buffer) and compared with an HA-specific antibody. Lane B: (0.5 µg/mL) staining of WT and KO lysates of Mouse Heart (100 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.
Product Details
PA5-37885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-HRRSMPVDERDLQ, from the internal region of RRAD (aa36-48)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2554493
Product Specific Information
This antibody is predicted to react with human, rat, dog, pig and cow based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
The Ras-encoded family of proteins bind GDP and GTP with high affinity. They possess a low level of intrinsic GTPase activity that increases more than 100-fold when interacting with cytosolic GTPase activating protein (GAP). Ras family members include H-Ras, K-Ras, N-Ras, M-Ras, R-Ras, ERas, Rheb, TC 21, RASL11B and Rad (Ras associated with diabetes) GTPase.Rad GTPase is a GTP-binding protein that is similar to Ras but has unique features. Unlike other small GTPases, Rad GTPase lacks typical pren-ylation motifs at its C terminus. The Rad GTPase enzyme binds calmodulin, inhibits vascular lesion formation, has low intrinsic GTPase activity and cannot be stimulated by any known GAP molecules. Rad GTPase is expressed in skeletal muscle, cardiac muscle and lung tissues and is overexpressed in the skeletal muscle tissue of individuals with type II diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GTP-binding protein RAD; Ras-like GTP-binding protein rad
Gene Aliases: Rad; REM3
Entrez Gene ID: (Mouse) 56437
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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