Invitrogen

RUNX1 Monoclonal Antibody (RXDMC), PE, eBioscience™

2 Published Figures
2 References
View all (44) RUNX1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

12-9816-80

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RXDMC

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151519

Product Specific Information

Description: This RXDMC monoclonal antibody reacts with human and mouse Runx1, which is also known as AML1. This 50-kDa transcription factor is expressed in hematopoietic cells such as myeloid, T, and B cells, but not erythroid cells. Furthermore, Runx1 can be detected in nearly all non-hematopoietic tissues except the brain and heart. Runx1 is involved in several stages of T cell development, including the CD4-CD8- double negative (DN) to CD4+CD8+ double positive (DP) transition and commitment to the CD4 lineage. In addition to its role in thymocyte development, Runx1 has also been implicated in Th2 differentiation and immune homeostasis via direct interaction with Foxp3 in CD4+CD25+ regulatory T cells. Runx1 also affects Th2 differentiation by negatively regulating Gata-3 expression. Finally, the AML1 gene, which encodes Runx1, is a frequent target of translocations in acute myeloid leukemia.

In humans, alternative splicing results in the generation of eleven isoforms. In mice, five isoforms exist as a result of alternative splicing; isoform 4 (aka AML1-C) is the most highly expressed in hematopoietic cells. Sequence analysis indicates that the RXDMC antibody recognizes isoform 4 in mouse and all human isoforms except AML-1FA, AML-1FB, and AML-1FC.

Applications Reported: This RXDMC antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This RXDMC antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor

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Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; EVI-1; Pebp2a2; PEBP2aB; PEBP2alpha; Pebpa2b; RUNX1

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UniProt ID: (Human) Q01196

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Entrez Gene ID: (Human) 861, (Mouse) 12394

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Function(s)
regulatory region DNA binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding ATP binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding protein homodimerization activity protein heterodimerization activity repressing transcription factor binding
Process(es)
ossification chondrocyte differentiation transcription from RNA polymerase II promoter hemopoiesis positive regulation of granulocyte differentiation positive regulation of interleukin-2 production positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter peripheral nervous system neuron development hematopoietic stem cell proliferation skeletal system development in utero embryonic development liver development myeloid progenitor cell differentiation regulation of T cell anergy transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter central nervous system development negative regulation of cell proliferation regulation of signal transduction myeloid cell differentiation neuron differentiation negative regulation of granulocyte differentiation hair follicle morphogenesis response to retinoic acid positive regulation of interferon-gamma production embryonic hemopoiesis negative regulation of transcription, DNA-templated behavioral response to pain neuron development definitive hemopoiesis regulation of hair follicle cell proliferation cellular response to transforming growth factor beta stimulus positive regulation of cell maturation positive regulation of progesterone secretion
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