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          • Primary Antibodies ›
          • RUNX2 Antibodies

          Abnova

          RUNX2 Monoclonal Antibody (2B9)

          View all (42) RUNX2 antibodies

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          Cite RUNX2 Monoclonal Antibody (2B9)

          • Antibody Testing Data (1)
          RUNX2 Antibody in Western Blot (WB)
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          RUNX2 Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          RUNX2 Antibody (H00000860-M02J) in WB

          RUNX2 monoclonal antibody (M02J), clone 2B9. Western Blot analysis of RUNX2 expression in PC-12. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RUNX2 Antibody in Western Blot (WB)

          Product Details

          H00000860-M02J

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          10-100 µg/mL
          -

          ELISA (ELISA)

          10 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Rat

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2B9

          Immunogen

          RUNX2 (NP_004339, 251 a.a. approximately 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          Product Specific Information

          Product may be used with Western Blot (Cell lysate).

          Immunogen sequence: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA

          Target Information

          RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Acute myeloid leukemia 3 protein; AML-3; CBF-alpha-1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt related transcription factor 2; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

          View more View less

          Gene Aliases: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2A; PEBP2aA; RUNX2

          View more View less

          UniProt ID: (Human) Q13950

          View more View less

          Entrez Gene ID: (Human) 860, (Rat) 367218

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding ATP binding protein domain specific binding histone deacetylase binding bHLH transcription factor binding repressing transcription factor binding DNA binding transcription factor binding transcription regulatory region DNA binding
          Process(es)
          skeletal system development osteoblast differentiation endochondral ossification osteoblast fate commitment chondrocyte differentiation chondrocyte development osteoblast development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of gene expression T cell differentiation regulation of ossification BMP signaling pathway positive regulation of chondrocyte differentiation embryonic forelimb morphogenesis regulation of fibroblast growth factor receptor signaling pathway odontogenesis of dentin-containing tooth regulation of odontogenesis of dentin-containing tooth cellular response to fibroblast growth factor stimulus regulation of osteoblast differentiation positive regulation of osteoblast differentiation positive regulation of ossification negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation embryonic cranial skeleton morphogenesis skeletal system morphogenesis stem cell differentiation cellular response to BMP stimulus positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus ossification regulation of transcription from RNA polymerase II promoter hemopoiesis neuron differentiation regulation of cell differentiation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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