Invitrogen

RUNX2 Recombinant Rabbit Monoclonal Antibody (6H4L27)

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2 Published Figures
1 Reference
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

702489

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:1,000
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

6H4L27

Immunogen

Protein corresponding to human RUNX2 [aa235-aa400]
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2725282

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Mouse, Rat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Acute myeloid leukemia 3 protein; AML-3; CBF-alpha-1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

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Gene Aliases: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2A; PEBP2aA; RUNX2

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UniProt ID: (Human) Q13950

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Entrez Gene ID: (Human) 860

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding ATP binding protein domain specific binding histone deacetylase binding bHLH transcription factor binding repressing transcription factor binding
Process(es)
ossification osteoblast differentiation endochondral ossification osteoblast fate commitment chondrocyte differentiation chondrocyte development osteoblast development regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation hemopoiesis neuron differentiation T cell differentiation BMP signaling pathway positive regulation of chondrocyte differentiation embryonic forelimb morphogenesis regulation of fibroblast growth factor receptor signaling pathway odontogenesis of dentin-containing tooth regulation of odontogenesis of dentin-containing tooth cellular response to fibroblast growth factor stimulus regulation of cell differentiation positive regulation of osteoblast differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated cell maturation embryonic cranial skeleton morphogenesis stem cell differentiation cellular response to BMP stimulus positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
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