Invitrogen

RUNX2 Recombinant Rabbit Monoclonal Antibody (6H4L27), Alexa Fluor™ Plus 647

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

702489RP647

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Flow Cytometry (Flow)

0.25-0.5 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

6H4L27

Immunogen

Protein corresponding to human RUNX2 [aa235-aa400]
View immunogen

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

proprietary buffer, pH 6.8

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant rabbit antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This 6H4L27 antibody has been tested by western blot and flow cytometric analysis of U-2 OS cells. This may be used for western blot at 5 µg/ml and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acute myeloid leukemia 3 protein; AML-3; CBF-alpha-1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

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Gene Aliases: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2A; PEBP2aA; RUNX2

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UniProt ID: (Human) Q13950

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Entrez Gene ID: (Human) 860

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding ATP binding protein domain specific binding histone deacetylase binding bHLH transcription factor binding repressing transcription factor binding Runt transcription factor
Process(es)
ossification osteoblast differentiation endochondral ossification osteoblast fate commitment chondrocyte differentiation chondrocyte development osteoblast development regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation hemopoiesis neuron differentiation T cell differentiation BMP signaling pathway positive regulation of chondrocyte differentiation embryonic forelimb morphogenesis regulation of fibroblast growth factor receptor signaling pathway odontogenesis of dentin-containing tooth regulation of odontogenesis of dentin-containing tooth cellular response to fibroblast growth factor stimulus regulation of cell differentiation positive regulation of osteoblast differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated cell maturation embryonic cranial skeleton morphogenesis stem cell differentiation cellular response to BMP stimulus positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
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