Invitrogen

RXRB Polyclonal Antibody

Product Details

PA1-815

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2182763

Product Specific Information

PA1-815 detects retinoid X receptor (RXR) beta from human and mouse cells as well as transfected human and mouse RXR beta.

PA1-815 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from COS cells transfected with the human gene. Gel shift assays reveal supershifting of vitamin D receptor (VDR)/RXR beta/vitamin D receptor response element (VDRE) complexes when PA1-815 is preincubated with RXR beta prior to addition of VDR.

The PA1-815 immunogen is a synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta.

Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.

Target Information

This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Thymocyte responsiveness to endogenous glucocorticoids is required for immunological fitness.

The Journal of clinical investigation

Mittelstadt PR,Monteiro JP,Ashwell JD

PA1-815 was used in western blot to study the importance of thymocyte responsiveness to endogenous glucocorticoids in immunological fitness

Sun Jul 01 00:00:00 UTC 2012

Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.

Journal of molecular endocrinology

Nygård M,Becker N,Demeneix B,Pettersson K,Bondesson M

PA1-815 was used in ChIP assay to investigate the effect of thyroid hormone on Necdin expression

Thu Jun 01 00:00:00 UTC 2006

The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.

Psychiatry research

Rioux L,Arnold SE

PA1-815 was used in immunohistochemistry to study the expression of retinoic acid receptor alpha in the granule cells of schizophrenic dentate gyrus

Sun Jan 30 00:00:00 UTC 2005

Retinoid x receptor agonists increase bcl2a1 expression and decrease apoptosis of naive T lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Rasooly R,Schuster GU,Gregg JP,Xiao JH,Chandraratna RA,Stephensen CB

PA1-815 was used in western blot to identify genes differentially regulated by the RXR agonist AGN194204 during primary antigenic stimulation of naive CD4+ T lymphocytes .

Thu Dec 15 00:00:00 UTC 2005

Induction of ovarian cancer cell apoptosis by 1,25-dihydroxyvitamin D3 through the down-regulation of telomerase.

The Journal of biological chemistry

Jiang F,Bao J,Li P,Nicosia SV,Bai W

PA1-815 was used in EMSA to study the role of 1,25(OH)2VD3 in regulating telomerase activity and cell death in ovarian epithelial cancer cells.

Fri Dec 17 00:00:00 UTC 2004

Expression patterns of retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos.

Biology of reproduction

Mohan M,Malayer JR,Geisert RD,Morgan GL

PA1-815 was used in immunohistochemistry to profile retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos

Fri Mar 01 00:00:00 UTC 2002

Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.

The Journal of biological chemistry

Thompson PD,Jurutka PW,Haussler CA,Whitfield GK,Haussler MR

PA1-815 was used in EMSA assay to investigate the effect of 1,25-(OH)2D3 and 9-cis-RA on the binding of VDR and RXR with VDREs

Fri Apr 03 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: DADB-100D22.1; MGC1831; MHC class I promoter binding protein; MHC class I regulatory element-binding protein H-2RIIBP; Nuclear receptor subfamily 2 group B member 2; putative retinoid X receptor beta; Retinoic acid receptor RXR-beta; retinoid X receptor; Retinoid X receptor beta; retinoid X receptor, beta; RXR Beta

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Gene Aliases: AL023085; DAUDI6; H-2RIIBP; NR2B2; RCoR-1; Rub; RXRB

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UniProt ID: (Human) P28702, (Mouse) P28704

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Entrez Gene ID: (Human) 6257, (Mouse) 20182

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding 9-cis retinoic acid receptor activity protein binding zinc ion binding thyroid hormone receptor coactivator activity vitamin D receptor binding retinoic acid receptor binding thyroid hormone receptor binding protein heterodimerization activity RNA polymerase II distal enhancer sequence-specific DNA binding DNA binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding

Process(es)

transcription initiation from RNA polymerase II promoter regulation of myelination steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter retinoic acid receptor signaling pathway positive regulation of receptor activity in utero embryonic development maternal placenta development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter ventricular cardiac muscle cell differentiation cardiac muscle cell proliferation cellular response to retinoic acid

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RXRB Polyclonal Antibody

Product Details

PA1-815

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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