Proteintech

RYR2 Polyclonal Antibody

RYR2 Antibody in Immunocytochemistry (ICC/IF)

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RYR2 Antibody (19765-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

RYR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RYR2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

19765-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detects a band at ~250 kDa which has been reported.

Target Information

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis.

Molecular oncology

Chen T,Zhang X,Ding X,Feng J,Zhang X,Xie D,Wang X

Sat Apr 01 00:00:00 UTC 2023

Dopamine receptor D4 (DRD4) negatively regulates UCP1- and ATP-dependent thermogenesis in 3T3-L1 adipocytes and C2C12 muscle cells.

Pflugers Archiv : European journal of physiology

Haddish K,Yun JW

Thu Jun 01 00:00:00 UTC 2023

Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage.

Human reproduction (Oxford, England)

Wang X,Shi W,Zhao S,Gong D,Li S,Hu C,Chen ZJ,Li Y,Yan J

Tue May 02 00:00:00 UTC 2023

Oxidative stress activates Ryr2-Ca²⁺ and apoptosis to promote PM_2.5-induced heart injury of hyperlipidemia mice.

Ecotoxicology and environmental safety

Meng M,Jia R,Wei M,Meng X,Zhang X,Du R,Sun W,Wang L,Song L

Tue Mar 01 00:00:00 UTC 2022

Elevated Ca²⁺ at the triad junction underlies dysregulation of Ca²⁺ signaling in dysferlin-null skeletal muscle.

Frontiers in physiology

Lukyanenko V,Muriel J,Garman D,Breydo L,Bloch RJ

Tue Aug 13 00:00:00 UTC 2024

Direct Cardiac Actions of the Sodium Glucose Co-Transporter 2 Inhibitor Empagliflozin Improve Myocardial Oxidative Phosphorylation and Attenuate Pressure-Overload Heart Failure.

Journal of the American Heart Association

Li X,Lu Q,Qiu Y,do Carmo JM,Wang Z,da Silva AA,Mouton A,Omoto ACM,Hall ME,Li J,Hall JE

Tue Mar 16 00:00:00 UTC 2021

IL-6/STAT3 Signaling Promotes Cardiac Dysfunction by Upregulating FUNDC1-Dependent Mitochondria-Associated Endoplasmic Reticulum Membranes Formation in Sepsis Mice.

Frontiers in cardiovascular medicine

Jiang T,Peng D,Shi W,Guo J,Huo S,Men L,Zhang C,Li S,Lv J,Lin L

Sun May 01 00:00:00 UTC 2022

SERCA2a ameliorates cardiomyocyte T-tubule remodeling via the calpain/JPH2 pathway to improve cardiac function in myocardial ischemia/reperfusion mice.

Scientific reports

Wang S,Zhou Y,Luo Y,Kan R,Chen J,Xuan H,Wang C,Chen J,Xu T,Li D

Thu Jan 21 00:00:00 UTC 2021

Applying cooperative module pair analysis to uncover compatibility mechanism of Fangjis: An example of Wenxin Keli decoction.

Journal of ethnopharmacology

Wang P,He T,Zheng R,Sun Y,Qiu R,Zhang X,Xing Y,Shang H

Tue Oct 05 00:00:00 UTC 2021

Endoplasmic Reticulum Stress/Ca²⁺-Calmodulin-Dependent Protein Kinase/Signal Transducer and Activator of Transcription 3 Pathway Plays a Role in the Regulation of Cellular Zinc Deficiency in Myocardial Ischemia/Reperfusion Injury.

Frontiers in physiology

Zhao H,Liu D,Yan Q,Bian X,Yu J,Wang J,Cheng X,Xu Z

Tue Jan 25 00:00:00 UTC 2022

Fibroblast Growth Factor 21 Protects Against Atrial Remodeling via Reducing Oxidative Stress.

Frontiers in cardiovascular medicine

Chen M,Zhong J,Wang Z,Xu H,Chen H,Sun X,Lu Y,Chen L,Xie X,Zheng L

Sat Oct 30 00:00:00 UTC 2021

Loss of Camk2n1 aggravates cardiac remodeling and malignant ventricular arrhythmia after myocardial infarction in mice via NLRP3 inflammasome activation.

Free radical biology & medicine

Wei Z,Fei Y,Wang Q,Hou J,Cai X,Yang Y,Chen T,Xu Q,Wang Y,Li YG

Sat May 01 00:00:00 UTC 2021

Arsenic (III) and/or Antimony (III) induced disruption of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in mice heart.

Ecotoxicology and environmental safety

Jiang X,Yu W,Wu S,Tang L,Zhong G,Wan F,Lan J,Zhang H,Pan J,Tang Z,Zhang X,Hu L,Huang R

Wed Sep 01 00:00:00 UTC 2021

Astragaloside IV attenuates chronic intermittent hypoxia-induced myocardial injury by modulating Ca²⁺ homeostasis.

Cell biochemistry and function

Jiang S,Jiao G,Chen Y,Han M,Wang X,Liu W

Sat Aug 01 00:00:00 UTC 2020

Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes.

Particle and fibre toxicology

Ju S,Lim L,Jiao HY,Choi S,Jun JY,Ki YJ,Choi DH,Lee JY,Song H

Thu Jun 11 00:00:00 UTC 2020

Tachycardia-induced CD44/NOX4 signaling is involved in the development of atrial remodeling.

Journal of molecular and cellular cardiology

Chen WJ,Chang SH,Chan YH,Lee JL,Lai YJ,Chang GJ,Tsai FC,Yeh YH

Tue Oct 01 00:00:00 UTC 2019

The harmful effects of acute PM_2.5 exposure to the heart and a novel preventive and therapeutic function of CEOs.

Scientific reports

Dong L,Sun W,Li F,Shi M,Meng X,Wang C,Meng M,Tang W,Liu H,Wang L,Song L

Tue Mar 05 00:00:00 UTC 2019

The harmful effects of acute PM_2.5 exposure to the heart and a novel preventive and therapeutic function of CEOs.

Scientific reports

Dong L,Sun W,Li F,Shi M,Meng X,Wang C,Meng M,Tang W,Liu H,Wang L,Song L

Tue Mar 05 00:00:00 UTC 2019

Atrial fibrillation and its arrhythmogenesis associated with insulin resistance.

Cardiovascular diabetology

Chan YH,Chang GJ,Lai YJ,Chen WJ,Chang SH,Hung LM,Kuo CT,Yeh YH

Thu Sep 26 00:00:00 UTC 2019

Protective role of heme oxygenase-1 in atrial remodeling.

Basic research in cardiology

Yeh YH,Hsu LA,Chen YH,Kuo CT,Chang GJ,Chen WJ

Thu Sep 01 00:00:00 UTC 2016

Protective role of heme oxygenase-1 in atrial remodeling.

Basic research in cardiology

Yeh YH,Hsu LA,Chen YH,Kuo CT,Chang GJ,Chen WJ

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: calcium release channel; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; cardiac-type ryanodine receptor; hRYR-2; islet-type ryanodine receptor; kidney-type ryanodine receptor; Ryanodine receptor; Ryanodine receptor 2; ryanodine receptor 2 (cardiac); ryanodine receptor type 2; RYR-2; Type 2 ryanodine receptor

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Gene Aliases: 9330127I20Rik; ARVC2; ARVD2; RyR; RYR-2; RYR2; VTSIP

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UniProt ID: (Human) Q92736, (Mouse) E9Q401

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Entrez Gene ID: (Human) 6262, (Mouse) 20191

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Function(s)

ryanodine-sensitive calcium-release channel activity calcium channel activity calcium ion binding protein binding calmodulin binding calcium-release channel activity enzyme binding protein kinase A catalytic subunit binding protein kinase A regulatory subunit binding identical protein binding protein self-association suramin binding ion channel binding calcium-induced calcium release activity ion channel activity protein kinase binding scaffold protein binding organic cyclic compound binding

Process(es)

response to hypoxia regulation of heart rate embryonic heart tube morphogenesis left ventricular cardiac muscle tissue morphogenesis cardiac muscle hypertrophy detection of calcium ion calcium ion transport cellular calcium ion homeostasis positive regulation of heart rate regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of cardiac muscle contraction by calcium ion signaling release of sequestered calcium ion into cytosol by sarcoplasmic reticulum response to muscle activity calcium-mediated signaling BMP signaling pathway response to caffeine ion transmembrane transport calcium-mediated signaling using intracellular calcium source response to muscle stretch release of sequestered calcium ion into cytosol positive regulation of sequestering of calcium ion regulation of cytosolic calcium ion concentration response to redox state regulation of cardiac muscle contraction cardiac muscle contraction canonical Wnt signaling pathway calcium ion transport into cytosol sarcoplasmic reticulum calcium ion transport cellular response to caffeine cellular response to epinephrine stimulus establishment of protein localization to endoplasmic reticulum ventricular cardiac muscle cell action potential Purkinje myocyte to ventricular cardiac muscle cell signaling cell communication by electrical coupling involved in cardiac conduction type B pancreatic cell apoptotic process positive regulation of the force of heart contraction regulation of AV node cell action potential regulation of SA node cell action potential regulation of atrial cardiac muscle cell action potential regulation of ventricular cardiac muscle cell action potential positive regulation of calcium-transporting ATPase activity regulation of cardiac conduction transport ion transport multicellular organism development response to magnesium ion negative regulation of cytosolic calcium ion concentration response to calcium ion transmembrane transport cytosolic calcium ion transport calcium ion transmembrane transport manganese ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RYR2 Polyclonal Antibody

RYR2 Antibody (19765-1-AP) in ICC/IF

Product Details

19765-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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