Invitrogen

Rab4 Polyclonal Antibody

Rab4 Antibody in Immunocytochemistry (ICC/IF)

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Rab4 Antibody (PA3-912) in ICC/IF

Immunofluorescence analysis of RAB4A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB4A Rabbit polyclonal Antibody (Product # PA3-912) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

Rab4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Rab4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

Rab4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAB4A specific validated siRNA (Silencer® select Product # s11675) and decrease in signal intensity was observed in western blot application using Anti-RAB4A Rabbit Polyclonal Antibody (Product # PA3-912). {KD}

Product Details

PA3-912

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:250

View 6 publications 6 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Bovine, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified, recombinant human Rab4 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2269382

Product Specific Information

PA3-912 detects Rab4 from human and mouse samples. This antibody does not detect other Rab isoforms (Rab1, Rab3, Rab5, or Rab6).

PA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~24 kDa band representing Rab4 from transfected HeLa cells.

The PA3-912 immunogen is purified recombinant human Rab4 protein.

Target Information

Protein transport. Probably involved in vesicular traffic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RAB5A expression is a predictive biomarker for trastuzumab emtansine in breast cancer.

Nature communications

Engebraaten O,Yau C,Berg K,Borgen E,Garred Ø,Berstad MEB,Fremstedal ASV,DeMichele A,Veer LV',Esserman L,Weyergang A

Published figure using Rab4 polyclonal antibody (Product # PA3-912) in Western Blot

Fri Nov 05 00:00:00 UTC 2021

Nuclear and Cytoplasmatic Quantification of Unconjugated, Label-Free Locked Nucleic Acid Oligonucleotides.

Nucleic acid therapeutics

Pendergraff H,Schmidt S,Vikeså J,Weile C,Øverup C,W Lindholm M,Koch T

PA3-912 was used in Western Blot to show compound variability (diversity) by which 247,000-693,000 LNAs/nuclei results in similar target reduction for different compounds.

Sat Feb 01 00:00:00 UTC 2020

Nuclear and Cytoplasmatic Quantification of Unconjugated, Label-Free Locked Nucleic Acid Oligonucleotides.

Nucleic acid therapeutics

Pendergraff H,Schmidt S,Vikeså J,Weile C,Øverup C,W Lindholm M,Koch T

PA3-912 was used in Western Blot to show compound variability (diversity) by which 247,000-693,000 LNAs/nuclei results in similar target reduction for different compounds.

Sat Feb 01 00:00:00 UTC 2020

Endosomal trafficking defects in patient cells with KIAA1109 biallelic variants.

Genes & diseases

Kane MS,Diamonstein CJ,Hauser N,Deeken JF,Niederhuber JE,Vilboux T

PA3-912 was used in Immunocytochemistry-immunoflourescence to study the mechanism by which KIAA1109 loss of function causes this spectrum of congenital neurological malformation disorders.

Fri Mar 01 00:00:00 UTC 2019

Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Deissler HL,Lang GK,Lang GE

PA3-912 was used in Immunocytochemistry to study aflibercept's associations with proteins either involved in neonatal Fc receptor-mediated transport or in the lysosomal pathway.

Tue Jan 01 00:00:00 UTC 2019

Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Deissler HL,Lang GK,Lang GE

PA3-912 was used in Immunocytochemistry to study aflibercept's associations with proteins either involved in neonatal Fc receptor-mediated transport or in the lysosomal pathway.

Tue Jan 01 00:00:00 UTC 2019

WDFY2 restrains matrix metalloproteinase secretion and cell invasion by controlling VAMP3-dependent recycling.

Nature communications

Sneeggen M,Pedersen NM,Campsteijn C,Haugsten EM,Stenmark H,Schink KO

Published figure using Rab4 polyclonal antibody (Product # PA3-912) in Immunocytochemistry

Fri Jun 28 00:00:00 UTC 2019

Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.

Nature cell biology

O'Farrell F,Lobert VH,Sneeggen M,Jain A,Katheder NS,Wenzel EM,Schultz SW,Tan KW,Brech A,Stenmark H,Rusten TE

PA3-912 was used in Immunocytochemistry-immunofluorescence to define a CIII-PI3K-regulated endosomal signalling platform from which LKB1 directs epithelial polarity, the dysregulation of which endows LKB1 with tumour-promoting properties.

Fri Dec 01 00:00:00 UTC 2017

Genome-wide, high-content siRNA screening identifies the Alzheimer's genetic risk factor FERMT2 as a major modulator of APP metabolism.

Acta neuropathologica

Chapuis J,Flaig A,Grenier-Boley B,Eysert F,Pottiez V,Deloison G,Vandeputte A,Ayral AM,Mendes T,Desai S,Goate AM,Kauwe JSK,Leroux F,Herledan A,Demiautte F,Bauer C,Checler F,Petersen RC,Blennow K,Zetterberg H,Minthon L,Van Deerlin VM,Lee VM,Shaw LM,Trojanowski JQ,Albert M,Moghekar A,O'Brien R,Peskind ER,Malmanche N,Schellenberg GD,Dourlen P,Song OR,Cruchaga C,Amouyel P,Deprez B,Brodin P,Lambert JC

Published figure using Rab4 polyclonal antibody (Product # PA3-912) in Immunofluorescence

Thu Jun 01 00:00:00 UTC 2017

Genome-wide, high-content siRNA screening identifies the Alzheimer's genetic risk factor FERMT2 as a major modulator of APP metabolism.

Acta neuropathologica

Published figure using Rab4 polyclonal antibody (Product # PA3-912) in Immunofluorescence

Thu Jun 01 00:00:00 UTC 2017

Dynamic internalization and recycling of a metal ion transporter: Cu homeostasis and CTR1, the human Cu⁺ uptake system.

Journal of cell science

Clifford RJ,Maryon EB,Kaplan JH

PA3-912 was used in immunocytochemistry study how importation of copper into cells is regulated

Fri Apr 15 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: HTLV-1 related endogenous sequence; Ras-related protein Rab-4A; Ras-related small GTP binding protein 4

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Gene Aliases: AI848268; HRES-1; HRES-1/RAB4; HRES1; RAB4; RAB4A

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UniProt ID: (Human) P20338, (Rat) P05714, (Mouse) P56371

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Entrez Gene ID: (Human) 5867, (Dog) 479210, (Rat) 25532, (Mouse) 19341

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Function(s)

ATPase activator activity GTPase activity protein binding GTP binding protein transporter activity GDP binding syntaxin binding ionotropic glutamate receptor binding ATPase binding nucleotide binding

Process(es)

protein transport antigen processing and presentation regulation of endocytosis Rab protein signal transduction positive regulation of ATPase activity intracellular protein transport small GTPase mediated signal transduction metabolic process transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Rab4 Polyclonal Antibody

Rab4 Antibody (PA3-912) in ICC/IF

Product Details

PA3-912

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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