Proteintech

RabGEF1 Polyclonal Antibody

RabGEF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

RabGEF1 Antibody (12735-1-AP) in ICC/IF

Immunofluorescent analysis of PC-3 cells using 12735-1-AP (RabGEF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RabGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

12735-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RabGEF1 Fusion Protein Ag3442 (136-491 aa encoded by BC015330)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DRVSKEFIE FLKTFHKTGQ EIYKQTKLFL EGMHYKRDLS IEEQSECAQD FYHNVAERMQ TRGKVPPERV EKIMDQIEKY IMTRLYKYVF CPETTDDEKK DLAIQKRIRA LRWVTPQMLC VPVNEDIPEV SDMVVKAITD IIEMDSKRVP RDKLACITKC SKHIFNAIKI TKNEPASADD FLPTLIYIVL KGNPPRLQSN IQYITRFCNP SRLMTGEDGY YFTNLCCAVA FIEKLDAQSL NLSQEDFDRY MSGQTSPRKQ EAESWSPDAC LGVKQMYKNL DLLSQLNERQ ERIMNEAKKL EKDLIDWTDG IAREVQDIVE KYPLEIKPPN QPLAAIDSEN VENDKLPPPL QPQVYAG (136-491 aa encoded by BC015330 )

Target Information

RABGEF1 forms a complex with rabaptin-5 (RABPT5) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vps9d1 regulates tubular endosome formation through specific activation of Rab22A.

Journal of cell science

Nakashima S,Matsui T,Fukuda M

Wed Mar 15 00:00:00 UTC 2023

LncRNA TM1-3P Regulates Proliferation, Apoptosis and Inflammation of Fibroblasts in Osteoarthritis through miR-144-3p/ONECUT2 Axis.

Orthopaedic surgery

Yi Y,Yang N,Yang Z,Tao X,Li Y

Tue Nov 01 00:00:00 UTC 2022

RabGEF1 functions as an oncogene in U251 glioblastoma cells and is involved in regulating AKT and Erk pathways.

Experimental and molecular pathology

Fan H,Xin T,Dong X,Yang F,Zhang R,Feng S,He D,Guo H,Pang Q

Mon Feb 01 00:00:00 UTC 2021

Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.

Cell reports

Pensalfini A,Kim S,Subbanna S,Bleiwas C,Goulbourne CN,Stavrides PH,Jiang Y,Lee JH,Darji S,Pawlik M,Huo C,Peddy J,Berg MJ,Smiley JF,Basavarajappa BS,Nixon RA

Tue Nov 24 00:00:00 UTC 2020

AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.

Scientific reports

Candiello E,Kratzke M,Wenzel D,Cassel D,Schu P

Thu Jul 14 00:00:00 UTC 2016

AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.

Scientific reports

Candiello E,Kratzke M,Wenzel D,Cassel D,Schu P

Thu Jul 14 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: RAB guanine nucleotide exchange factor (GEF) 1; Rab5 exchange factor; Rab5 GDP/GTP exchange factor; Rabaptin-5-associated exchange factor for Rab5; Rabex-5; RAP1; Ras negative regulator Rabex-5; Ras negative regulator Rabex-5/Rin2

View more View less

Gene Aliases: Rab5ef; rabex-5; RABEX5; RABGEF1; RAP1; Rin2

View more View less

UniProt ID: (Human) Q9UJ41, (Mouse) Q9JM13

View more View less

Entrez Gene ID: (Human) 27342, (Mouse) 56715, (Rat) 360797

View more View less

Function(s)

DNA binding protein binding zinc ion binding Rab guanyl-nucleotide exchange factor activity Rab GTPase binding ubiquitin protein ligase activity metal ion binding ubiquitin-protein transferase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

RabGEF1 Polyclonal Antibody

RabGEF1 Antibody (12735-1-AP) in ICC/IF

Product Details

12735-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!