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Invitrogen
Rabbit IgG Recombinant Isotype Control (SP137)
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Rabbit IgG Isotype Control (MA5-16384) in Flow
Flow Cytometry analysis of TLR4 in formaldehyde-fixed THP-1 cells (green) compared to an isotype control (blue). Staining was performed using a TLR4 polyclonal antibody (Product # PA5-23124) at a dilution of 20µg x 10^6 cells, followed by secondary detection using a Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate (Product # A-11008) at a dilution of 1:200. Rabbit IgG Isot... View More
Product Details
MA5-16384
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
1:100
Flow Cytometry (Flow)
20 µg x 10^6 cells
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Recombinant Monoclonal
Type
Isotype Control
Clone
SP137
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537903
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature.
Target Information
Rabbit IgG refers to immunoglobulin G antibodies produced by rabbits in response to an antigen. These antibodies are commonly used in laboratory research as a detection tool for specific proteins, molecules, or cells. Rabbit IgG antibodies are highly specific and have a strong affinity for their target, making them ideal for use in various applications such as western blotting, immunohistochemistry, and flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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