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Invitrogen
Rabbit anti-Sheep IgG (H+L) Secondary Antibody
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FIGURE: 1 / 3
Sheep IgG (H+L) Secondary Antibody (31240) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Sheep IgG (H+L) Secondary Antibody (Product # 31240) was performed using Hep G2 cells stained with CD49a Polyclonal Antibody (Product # PA5-47763). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 2% BSA for 1 hour and labeled with 1:100 dilution of primary antibody for 3 hours at room ... View More
Product Details
31240
Applications
Tested Dilution
Western Blot (WB)
1:2000-1:5000
Immunohistochemistry (IHC)
Assay-dependent
Immunocytochemistry (ICC/IF)
1:2000
Flow Cytometry (Flow)
Assay-dependent
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_228453
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31240 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31240 reacts with the heavy chains of sheep IgG and with the light chains common to most sheep immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
NOTE: For blocking, we recommend using a 4-5 % normal serum from the same host species of the labeled secondary antibody. The use of BSA and/or dry milk to block or dilute anti-bovine IgG, anti-goat IgG, anti-horse IgG, anti-sheep IgG and/or primary antibodies, may significantly increase background and/or reduce secondary antibody titer since BSA and dry milk may contain IgG which reacts with these antibodies.
Country of Origin: USA
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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