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Invitrogen
Ras (A59T Mutant) Polyclonal Antibody
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Ras (A59T Mutant) Antibody (PA5-78084) in WB
Western Blot analysis of Ras (A59T Mutant) was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Ras (A59T Mutant) Polyclonal Antibody (Product # PA5-78084) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-78084
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant A59T of human K-Ras. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736498
Product Specific Information
Positive Control: Human Ras (A59T Mutant specific)-transfected 293T whole cell extracts
Predicted Reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Chicken (100%), Bovine (100%), Guinea pig (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Ras is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. The process of shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in Ras gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTPase NRas; N-ras protein part 4; neuroblastoma RAS viral (v-ras) oncogene homolog; Transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
Gene Aliases: ALPS4; CMNS; HRAS1; N-ras; NCMS; NRAS; NRAS1; NS6
UniProt ID: (Human) P01111
Entrez Gene ID: (Human) 4893
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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